We narrowed to 15,050 results for: pET

1. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

   Plasmid

   #226350

   Purpose

   Expression of proteasome substrate with tail for molecular ruler

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM-60-NusA-TEV-HsRCK_296-472_E

   Plasmid

   #146209

   Purpose

   Bacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck_296-472 (DDX6 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HOXB8 (murine) HIS-tag pET

   Plasmid

   #8540

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB8 (Hoxb8 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   starts at amino acid #2 (Ser)

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-His10-SHP1_PTPase (243-541aa)

   Plasmid

   #180812

   Purpose

   Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   SHP1_PTPase (243-541aa) (PTPN6 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETNKI-his-SUMO-RNF168ring (1-189)

   Plasmid

   #63570

   Purpose

   Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   6xHis and SUMO3

   Expression

   Bacterial

   Mutation

   Ring domain only (residues 1-189)

   Promoter

   T7

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pET15b/GgVcl 884-1066-ECFP

   Plasmid

   #46182

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Tags

   ECFP and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET-His6-Sumo-MBP-CfaN

   Plasmid

   #126016

   Purpose

   Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

   Depositor

   Thomas Muir

   Insert

   His5-Sumo-MBP-CfaN
   

   Tags

   5xHis-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-Streptavidin-Dead Aspartate Loop

   Plasmid

   #46368

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Dead Aspartate Loop
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET-45b-RUNX2(WT)-IDR-mCherry

   Plasmid

   #145286

   Purpose

   Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   RUNX2(WT)-IDR (RUNX2 Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-28a-His6-SUMO-VAMP3

    Plasmid

    #135552

    Purpose

    Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP3
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET empty polycistronic destination vector (2Z)

    Plasmid

    #29776

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29b-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175783

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET15b/GgVcl 1-1066 (full length)

    Plasmid

    #46171

    Depositor

    Susan Craig

    Article

    Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

    Insert

    Vcl (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pET-45b-HOXD13-IDR-mCherry

    Plasmid

    #145280

    Purpose

    Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Tags

    His x10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21-10XHis-GST-HRV-dHer1cys

    Plasmid

    #73218

    Purpose

    Bacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    10XHis-GST-HRV-dHer1cys
    

    Tags

    10XHis-GST-HRV and The dimer of the Her1 Affibody…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET29-hMIGA2(170-593)-6xhis

    Plasmid

    #192834

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-His6-Sumo-IntNtr-eSrt2A

    Plasmid

    #126015

    Purpose

    Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-IntNtr-eSrt2A
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET15b-Casp8 delta DED C360A

    Plasmid

    #11828

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Minus Death Defecter Domain (aa 1-216), C360A

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid

    #185791

    Purpose

    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-sfGFP(S45C)-ZdK1
    

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Ser to Cys mutation at start of GFP, residue 45 i…

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HOXA10 (human) HIS-tag pET

    Plasmid

    #8516

    Depositor

    Corey Largman

    Article

    Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

    Insert

    HOXA10 (HOXA10 Human)
    

    Expression

    Bacterial

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

29. HOXA5 (human) HIS-tag pET

    Plasmid

    #8562

    Depositor

    Corey Largman

    Insert

    HOXA5 (HOXA5 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    SacI site at amino acids 1-2 was used to clone in…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

30. HOXC9 (murine) HIS-tag pET

    Plasmid

    #8559

    Depositor

    Corey Largman

    Insert

    HOXC9 (Hoxc9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only