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pCDNA3_dCas7-11

Plasmid

#197605

Purpose

Expresses inactive Cas7-11 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

dCas7-11

Expression

Mammalian

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAS168

Plasmid

#194491

Purpose

Zif268 Op (Part 2)

Depositor

Ahmad Khalil

Article

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

Zif268 Op (Part 2)

Use

Synthetic Biology

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p15aTetT7mut-fuseYFP

Plasmid

#28013

Depositor

Lingchong You

Article

Tan et al Nat Chem Biol. 2009 Nov . 5(11):842-8.

Insert

EYFP-fuse, T7 RNAP

Expression

Bacterial

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AbKRS-chIPYE

Plasmid

#140020

Purpose

Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AbKRS-chIPYE (pylS Methanosarcina mazei and Methanosarcina bakeri)

Tags

FLAG

Expression

Mammalian

Mutation

1-149 MbPylRS IPYE (V31I, T56P, H62Y, A100E) + 18…

Promoter

EF1

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUltra-FPheKRS

Plasmid

#136631

Purpose

Genetic Incorporation 4-fluorophenyl carbamate lysine (FPheK) into proteins

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

FPheKRS

Expression

Bacterial

Promoter

Tac1 promoter

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPROBE'-gfp[LVA]

Plasmid

#40171

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-SNAP-Smo

Plasmid

#31619

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Expression

Mammalian

Mutation

T130A L131S

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
RP hk339-GFP

Plasmid

#24431

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin WT (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

monomeric kinesin

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2 hSmad1-EVE-MM

Plasmid

#22994

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A, S463E, S465E

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

2xmyc

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCitrine-GFP11

Plasmid

#61667

Purpose

Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNO4

Plasmid

#101066

Purpose

BphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFP

Depositor

Jeffrey Tabor

Article

Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-Cdh1

Plasmid

#49776

Purpose

pENTR vector for mouse E-cadherin

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Cdh1 (Cdh1 Mouse)

Use

Gateway Cloning

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
XE100 Pertussis toxin A protomer

Plasmid

#16678

Depositor

Randall Moon

Article

Slusarski et al Nature. 1997 Nov 27;390(6658):410-3. doi

Insert

Pertussis toxin A protomer (ptxA Bordetella pertussis)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET28CLY2

Plasmid

#21761

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing two SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Nov. 30, 2009

Availability

Academic Institutions and Nonprofits only
HOXA11 (murine) HIS-tag pET

Plasmid

#8517

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXA11 (Hoxa11 Mouse)

Tags

T7

Expression

Bacterial

Mutation

makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only