We narrowed to 3,202 results for: Streptococcus

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pX330-Flag-dHeFSpCas9

Plasmid

#92116

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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MYC sgRNA5

Plasmid

#100557

Purpose

Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA5

Promoter

U6

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_ST1_2x_binding

Plasmid

#79368

Purpose

Assays activity of transcriptional activators fused to ST1 Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Expression

Mammalian

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRB1084

Plasmid

#71516

Purpose

Expresses gRNA for dpy-10 conversion using NGCG PAM and VRER Cas9 mutant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-HA-TMSec66-SrtA (pKS105)

Plasmid

#50033

Purpose

Galactose-inducible expression of ER-membrane S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Tags

HA

Expression

Yeast

Promoter

pGAL

Available Since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-HA-SrtAstrep (pKS78)

Plasmid

#50029

Purpose

Galactose-inducible expression of cytosolic S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Tags

HA

Expression

Yeast

Promoter

pGAL

Available Since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -126

Plasmid

#50924

Purpose

U6 driven sgRNA targeting Sox17 -126 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -126 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-NM!TB

Plasmid

#48652

Purpose

Bacterial NM crRNA expression: targets NM to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial NM crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-ST1!TB

Plasmid

#48654

Purpose

Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial ST1 crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-TD!TA

Plasmid

#48655

Purpose

Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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PM-SP!TA

Plasmid

#48649

Purpose

Bacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer A

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pIND20-KNL1-rvsf_aaaa-dCas9

Plasmid

#248562

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

TRE, miniCMV

Available Since

May 15, 2026

Availability

Academic Institutions and Nonprofits only

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SpyCas9-E566-LOV-C450A-D432V(M519)

Plasmid

#250779

Purpose

Potent temperature-deactivated SpyCas9

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

SpyCas9 with an AsLOV2 insertion behind E566 and C450A and D432V mutation

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

C450A, D432V

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pV1210

Plasmid

#242891

Purpose

Modified plasmid from pV1200 to perform ENO1-SI CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE-KNL1-s24a_s60a-dCas9

Plasmid

#248568

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-s24a_s60a-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 S24A, S60A; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9

Plasmid

#237460

Purpose

Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

3xFlag_ABE8e_SpCas9(D10A)_6xNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pSA073 HBB_IVS2-Cas9-BFP

Plasmid

#249134

Purpose

Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

HBB_IVS2-Cas9-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA071 Cas9-BFP

Plasmid

#249132

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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