We narrowed to 12,571 results for: lic

1. pDestTol2-actinb-kcnj13-EGFP

   Plasmid

   #164940

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Expression

   Mutation

   Stop codon deleted

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

   Plasmid

   #107163

   Purpose

   Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme

   Depositor

   Jose Carlos Cameselle

   Article

   Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.

   Insert

   F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDestTol2-pax3a-kcnj13-IRES-EGFP

   Plasmid

   #164950

   Purpose

   Tol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   pax3a promoter -5.4k

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2-actinb-kcnj13-IRES-EGFP

   Plasmid

   #164941

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-human-BNIP3 delta Exon2

   Plasmid

   #100782

   Purpose

   Mammalian expression of BNIP3 delta Exon2 splice variant

   Depositor

   Joseph Gordon

   Article

   Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

   Insert

   human-BNIP3 delta Exon2 (BNIP3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Exon2 deletion

   Promoter

   CMV

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. H14-MBP-SUMO-Cys-Linker-BAF_L58R

   Plasmid

   #101780

   Purpose

   Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Tags

   His14-MBP-SUMO

   Expression

   Bacterial

   Mutation

   L58R mutation

   Promoter

   T5

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES:mTREK-1(K271Q)

   Plasmid

   #133273

   Purpose

   Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

   Depositor

   Dan Minor

   Article

   Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian and Yeast

   Mutation

   K271Q

   Promoter

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-10-3xFLAG-LGP2-MIII

   Plasmid

   #58683

   Purpose

   Expresses LGP2 with a mutation to motif III in mammalian cells

   Depositor

   Curt Horvath

   Article

   Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

   Insert

   LGP2-MIII (DHX58 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   threonine and serine of helicase motif III mutate…

   Promoter

   CMV

   Available since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pLJC2-DHFR-3xFLAG

   Plasmid

   #217428

   Purpose

   Lentiviral overexpression of human DHFR

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   DHFR (DHFR Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Insert sequence is a codon-optimized gBLOCK (IDT)

   Promoter

   CMV

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR-v1-sgABCC4_1

    Plasmid

    #217433

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 1 targeting ABCC4 (ABCC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR-v1-sgABCC4_3

    Plasmid

    #217434

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    sgRNA 3 targeting ABCC4 (ABCC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid

    #226247

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA1_Cas9-hGem_Smc4

    Plasmid

    #217667

    Purpose

    expresses Cas9-hGem and guideRNA for tagging of SMC4

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Hygro-P2A-HA-FKBP-WAPL

    Plasmid

    #217666

    Purpose

    Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    WapI (WAPL Human)
    

    Use

    Tags

    dTag (FKBP36V protein)

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Lac

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid

    #189636

    Purpose

    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMMK ENTR 1 HITI-2c ACTB

    Plasmid

    #206269

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HITI-2c donor
    

    Use

    CRISPR; Multimate/gateway entr 1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. anti-Calbindin scFv [L109/57] with sortase tag

    Plasmid

    #204421

    Purpose

    Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting Calbindin (Homo sapiens)
    

    Use

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. anti-Parvalbumin scFv [L114/81] with sortase tag

    Plasmid

    #204423

    Purpose

    Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)
    

    Use

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. anti-Kv1.2 scFv [K14/16] with sortase tag

    Plasmid

    #204425

    Purpose

    Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.

    Depositor

    Jeff Lichtman

    Article

    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

    Insert

    Recombinant mouse scFv targeting Kv1.2 (Homo sapiens)
    

    Use

    Tags

    6xHis tag and Sortase tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Coexpressed VLP NES

    Plasmid

    #182433

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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