We narrowed to 22,815 results for: Fus
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Plasmid#167977PurposeExpresses fusion protein AID-T7RNAP in E. coliDepositorInsertAID-T7RNAP
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH233-Tier1-PmPGK1-eYFP
Plasmid#169522PurposeTier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).DepositorInsertPPGK-driven enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinE-His
Plasmid#133623PurposeExpression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.DepositorInsertMinE-His (minE E. coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCW007
Plasmid#83525Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha1
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYC46
Plasmid#63904PurposeRecipient integrative vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TrxA-AID-T7RNAP
Plasmid#167976PurposeExpresses fusion protein TrxA-AID-T7RNAP in E. coliDepositorInsertTrxA-AID-T7RNAP
UseTagsT7RNAP and TrxAExpressionBacterialMutationPromoterPtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC61
Plasmid#63925PurposeRecipient replicative vector to construct CFP tagged fusionsDepositorTypeEmpty backboneUseTagsCFPExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC55
Plasmid#63922PurposeRecipient replicative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC42
Plasmid#63920PurposeRecipient replicative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC48
Plasmid#63905PurposeRecipient integrative vector to construct HA tagged fusionsDepositorTypeEmpty backboneUseTagsHAExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC54
Plasmid#63909PurposeRecipient integrative vector to construct YFP tagged fusionsDepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC52
Plasmid#63908PurposeRecipient integrative vector to construct CFP tagged fusionsDepositorTypeEmpty backboneUseTagsCFPExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC50
Plasmid#63906PurposeRecipient integrative vector to construct MYC tagged fusionsDepositorTypeEmpty backboneUseTagsMYCExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVH234-Tier1-PmPGK1-tTA
Plasmid#169574PurposeTier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCW008
Plasmid#83526Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoterDepositorInsertpFUS1J2-mfalpha2
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYC64
Plasmid#63928PurposeRecipient replicative vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC62
Plasmid#63927PurposeRecipient replicative vector to construct HA tagged fusionsDepositorTypeEmpty backboneUseTagsHAExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBluescript Sp5
Plasmid#16884DepositorInsertsp5 (sp5 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114105PurposeCre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC66
Plasmid#63929PurposeRecipient replicative vector to construct MYC tagged fusionsDepositorTypeEmpty backboneUseTagsMYCExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVH235-Tier1-PmPGK1-rtTA
Plasmid#169578PurposeTier-1 vector encoding PmPGK1-driven rtTA expression (PmPGK1-rtTA-pA).DepositorInsertreverse tetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH230-2-Tier1-PmPGK1-YPet
Plasmid#169521PurposeTier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).DepositorInsertPPGK-driven yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB300-Tier1-PhCMV-rTetR
Plasmid#169577PurposeTier-1 vector encoding PhCMV-driven tetracycline-activated rTetR (PhCMV-rTetR-VPR-pA).DepositorInsertreverse tetracycline repressor protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2368-Tier1-PhCMV_VP64
Plasmid#169569PurposeTier-1 vector encoding PhCMV-driven VP64 transactivator domain (PhCMV-VP64-pA).DepositorInsertPCMV-driven VP64
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2367-Tier1-PhCMV_VP16
Plasmid#169568PurposeTier-1 vector encoding PhCMV-driven VP16 transactivator domain (PhCMV-VP16-pA).DepositorInsertPCMV-driven VP16
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1011-Tier1-PhCMV-Nluc
Plasmid#169545PurposeTier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).DepositorInsertPCMV-driven NanoLuc Luciferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFOX8-Tier1-PhCMV-YPet
Plasmid#169506PurposeTier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).DepositorInsertPCMV-driven yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2401-Tier1-PhCMV-HygroR
Plasmid#169493PurposeTier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).DepositorInsertPCMV-driven hph
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1018-Tier1-PhCMV-p2A
Plasmid#169491PurposeTier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).DepositorInsertPCMV-driven 2A sequence of porcine teschovirus-1
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2415-Tier1-PPGK-1xEAAAK
Plasmid#169482PurposeTier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).DepositorInsertPPGK-driven EAAAK-linker
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2412-Tier1-PPGK-2xGS
Plasmid#169481PurposeTier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).DepositorInsertPPGK-driven GS-linker
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL38-decoder
Plasmid#159452PurposeInsert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2DepositorInsertPfus1tet-LexA-ER-B112-tCyc1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfus1tetAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
UseTagsExpressionYeastMutationPromoterTefAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
UseTagsExpressionYeastMutationPromoterGPD1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(1-93).ER.VP16
Plasmid#108216PurposeYeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domainDepositorInsertGal4 DBD-ERalpha HBD-VP16
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH1Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
FsC_pET29b
Plasmid#215405PurposeExpression construct for FsC gene, codon optimized for expression in E. coliDepositorInsertFsC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH41-Tier1-PhCMV-GLuc
Plasmid#169544PurposeTier-1 vector encoding PhCMV-driven gaussia luciferase (PhCMV-GLuc-pA)DepositorInsertPCMV-driven Gaussia Luciferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2374-Tier1-PhCMV-Gal4_VP16
Plasmid#169582PurposeTier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).DepositorInsertGAL4-transactivator
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRα-β flap (831-1057)
Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHmK-C1
Plasmid#87866PurposeBackbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1DepositorInsertpHluorin-mKate2 fusion
UseTagspHluorin-mKate2 fusionExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-spCas9-SB
Plasmid#169919PurposeExpression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.DepositorInsertSpCas9-SB100X
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9-NED
Plasmid#109358PurposeMammalian vector for expressing effector domains fused to dCas9.DepositorTypeEmpty backboneUseCRISPRTagsdCas9, SV40 NLS, 3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS1017-Tier1-OTetO7-PCMVmin-SEAP
Plasmid#169586PurposeTier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVminAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only