We narrowed to 35,392 results for: CaS;

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CcuCas9

Plasmid

#192127

Purpose

Expresses CcuCas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

CcuCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain (K81A)

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp2Cas9

Plasmid

#192126

Purpose

Expresses Hsp2Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp2Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1Cas9

Plasmid

#192125

Purpose

Expresses Hsp1Cas9

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1Cas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

Plasmid

#195504

Purpose

Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG, Puro, and T2A

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS-mak10 gRNA

Plasmid

#160385

Purpose

Expresses guide RNA for targeting MAK10 in yeast

Depositor

John McCusker

Insert

mak10 gRNA

Expression

Yeast

Promoter

RNA pol III promoter (tRNA-Tyr)

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Uricase

Plasmid

#182416

Purpose

Bacterial expression of mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-uricase

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS.Sfi Akt1-K179M

Plasmid

#196375

Purpose

Akt1 kinase negative mutant K179M in avian expression vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

c-akt1 K179M

Use

Retroviral; Avian

Tags

HA

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS myr-c-P3k

Plasmid

#196373

Purpose

Oncogenic with addition of a myristylation signal and expresses in avian cells

Depositor

Peter Vogt

Article

Chang et al Science. 1997 Jun 20;276(5320):1848-50.

Insert

Myr-c-P3k-FLAG

Use

Retroviral; Avian

Tags

flag and myristoylation

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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CASK scFv [K56A/57]

Plasmid

#190492

Purpose

Mammalian Expression of CASK scFV. Derived from hybridoma K56A/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

CASK (Homo sapiens) recombinant scFV (CASK Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CASK scFv [K56A/50]

Plasmid

#190491

Purpose

Mammalian Expression of CASK scFV. Derived from hybridoma K56A/50.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

CASK (Homo sapiens) recombinant scFV (CASK Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-CASK [K56A/57]

Plasmid

#190283

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/57.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant Mouse monoclonal antibody (CASK Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUBI-RZ-Cas12j2

Plasmid

#189781

Purpose

Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

Plasmid

#183387

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

redesigned Caspase-4 (AA94-377) (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

ANR261ENP, D315H, M318K, T352K

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-puro-hCASP8

Plasmid

#185378

Purpose

For mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)

Depositor

Ayaz Najafov

Insert

CASP8 (CASP8 Human)

Expression

Mammalian

Mutation

WT

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15541_dCBE-SuperFi-Cas9

Plasmid

#184371

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dCBE-SuperFi-Cas9

Use

CRISPR

Tags

3X FLAG, SV40 NLS, and UGI

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15543_dABE-SuperFi-Cas9

Plasmid

#184373

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE-SuperFi-Cas9

Use

CRISPR

Tags

FLAG, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15545_dABE8e-SuperFi-Cas9

Plasmid

#184375

Purpose

Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

dABE8e-SuperFi-Cas9

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

Promoter

EF-1α core promoter

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-H2BC11_sgRNA

Plasmid

#183883

Purpose

pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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