We narrowed to 5,822 results for: pcas

1. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

   Plasmid

   #69223

   Purpose

   Expresses R1335K mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry

   Plasmid

   #89057

   Purpose

   PV4 for 2-input, 2-output circuit library

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   GFP-T2A-mCherry
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

   Plasmid

   #116956

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

   Plasmid

   #87574

   Purpose

   PV1 for 2-input, 4-output decoder circuit

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mtagBFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75233

   Purpose

   CRISPR/Cas9 plasmid against human NFATc2

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107322

   Purpose

   Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1335K) is fused to SaCas9

   Promoter

   CMV IE93

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

   Plasmid

   #242660

   Purpose

   CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette

   Depositor

   Benjamin Kleinstiver

   Insert

   pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
   

   Use

   AAV and CRISPR

   Tags

   BPNLS

   Mutation

   VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

   Promoter

   CBh

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

   Plasmid

   #242659

   Purpose

   CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs

   Depositor

   Benjamin Kleinstiver

   Insert

   pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and NpuN(intein)-BPNLS

   Mutation

   SpCas9(S55R)

   Promoter

   CBh

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

   Plasmid

   #242664

   Purpose

   CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   VQR mutations in SpCas9(D1135V/R1335Q/T1337R)

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-mVenus-rRim1[20-227]-mVenus(L68V)

    Plasmid

    #246280

    Purpose

    Rab10 sensor acceptor

    Depositor

    Ryohei Yasuda

    Article

    Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

    Insert

    Venus-rRim1[20-227]-mVenus(L68V) (Rims1 Rat)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-mCherry-FYCO1[963-1206]-mCherry

    Plasmid

    #246264

    Purpose

    Rab7 sensor acceptor

    Depositor

    Ryohei Yasuda

    Article

    Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

    Insert

    mCherry-FYCO1[963-1206]-mCherry (Fyco1 Rat)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-mCherry-Rabenosyn5[439-503]-mCherry

    Plasmid

    #246251

    Purpose

    Rab4 sensor acceptor

    Depositor

    Ryohei Yasuda

    Article

    Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

    Insert

    mCherry-Rabenosyn5[439-503]-mCherry (Zfyve20 Rat)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGEN-DV-IRES-myr-mKate (JDW 494)

    Plasmid

    #242575

    Purpose

    Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attR1-CmR-ccdB-attR2
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 6

    Plasmid

    #236623

    Purpose

    Expresses human ANKRD11 fragment in mammalian cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Tags

    3xFLAG and 3xHA

    Expression

    Mammalian

    Mutation

    aa 1831-2663

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 2

    Plasmid

    #236619

    Purpose

    Expresses human ANKRD11 fragment in mammalian cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Tags

    3xFLAG and 3xHA

    Expression

    Mammalian

    Mutation

    aa 1-1006

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1

    Plasmid

    #236618

    Purpose

    Expresses human ANKRD11 fragment in mammalian cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Tags

    3xFLAG and 3xHA

    Expression

    Mammalian

    Mutation

    aa 1-658

    Available Since

    Sept. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

    Plasmid

    #242589

    Purpose

    A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh (actin nanobody)
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-MBP-OSF-TEVcs-GFP-hULK1

    Plasmid

    #239015

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    hULK1 (ULK1 Human)
    

    Tags

    MBP-OSF-TEV-GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pCAG-Floxed-SynGFP,rev.-WPRE

    Plasmid

    #234892

    Purpose

    CAG-driven, Cre-dependent synaptophysin-tagged GFP for anterograde tracing of neuronal projections

    Depositor

    Silvia Arber

    Article

    Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

    Insert

    Floxed-SynGFP
    

    Use

    AAV

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)

    Plasmid

    #223102

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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