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pLenti-HIFR

Plasmid

#192946

Purpose

Lentiviral production of HIF-1alpha reporter

Depositor

Markus Covert

Article

Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

Insert

HIF-1alpha reporter

Use

Lentiviral

Promoter

mCMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Lyn11-FRB

Plasmid

#155228

Purpose

membrane anchor for FKBP-FRB system

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system

Expression

Mammalian

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCasTet-λ

Plasmid

#128318

Purpose

Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

Depositor

Christopher Johnston

Article

Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

Insert

TetR and pTetR/TetO

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Constitutive expression of cas9 and anhydro-tetra…

Promoter

tet promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJOE_pvdJ

Plasmid

#110142

Purpose

Suicide plasmid with repair template for knockout in P. putida

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Type

Empty backbone

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEVOL-pAzF[TAG]

Plasmid

#164579

Purpose

Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

araBAD, glnS, and proK

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

Plasmid

#194457

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

Plasmid

#192066

Purpose

Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Mouse Ctip2

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KB

Plasmid

#139310

Purpose

Recruitable PI4KB

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-LACTB-dAPEX2

Plasmid

#117179

Purpose

Plasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Pur-CAG-hM4Di-mCherry

Plasmid

#80947

Purpose

human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

Insert

hM4Di-mCherry

Tags

mcherry fusion

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKAR

Plasmid

#60445

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-Tox-IRES-eGFP

Plasmid

#165427

Purpose

Expresses TOX in mammalian cells

Depositor

Patrick Hogan, Anjana Rao

Article

Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

Insert

TOX (Tox Mouse)

Tags

3xflag 5' of Tox

Expression

Mammalian

Promoter

5' LTR

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRC10-EFS-huLbCpf1-2A-Blast-WPRE

Plasmid

#123359

Purpose

Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

Plasmid

#111818

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Mef2c (Mef2c Mouse)
Tdtomato
Gata4 (Gata4 Mouse)
Tbx5

Use

Cloning intermediate

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Myristoylated optoDroplet

Plasmid

#111507

Purpose

For membrane localized optoDroplets

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

Cry2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

FUS, Fusion Red, and Myristoylation Sequence (MYR…

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGT-B1

Plasmid

#122579

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTREX GPR68_NB2_2

Plasmid

#127691

Purpose

Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

GPR68_NB2_2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23-MYC

Plasmid

#86461

Purpose

A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

MYC

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T4 DNA Ligase

Plasmid

#206207

Purpose

Inducible expression of T4 DNA ligase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

T4 DNA ligase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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