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CAG_IGF1R_EGFP
Plasmid#199628PurposeFRET donor for IGF1R sensorDepositorInsertIGF1R (IGF1R Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_eYFP
Plasmid#188035PurposeExpresses CapChR2-eYFP in mammalian neuronsDepositorInsertCapChR2-eYFP
UseAAVTagseYFPExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-EGFP:LIMD1
Plasmid#108230PurposeLentiviral vector for Tet-inducible LIMD1 expressionDepositorInsertLIMD1 (LIMD1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-sh5Resistant
Plasmid#225448PurposeExpress mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutationDepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD2
Plasmid#85658Purposeretroviral expression of human SMYD2DepositorInsertSMYD2 (SMYD2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterLTRAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCry2PHR-mCh-RhoA
Plasmid#42958PurposeExpresses optogenetically controlled RhoADepositorInsertCry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-mp1
Plasmid#42329DepositorInsertmp1 (LAMTOR3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-SAKS1-Strep-HA
Plasmid#113487PurposeExpression of human SAKS1 with C-terminal strep-HA tagDepositorInsertSAKS1 (UBXN1 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-GIT1
Plasmid#15226DepositorInsertG protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
UseTagsGFPExpressionMammalianMutationL425V, E476K and L583PPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGfap-R-CaMP1.07-WPRE-SV40
Plasmid#164140PurposeAAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.DepositorInsertR-CaMP1.07
UseAAVTagsExpressionMutationPromoterhGFAPAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-NLS-EGFP-deadBtgh-3NLS
Plasmid#194470PurposeEncodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.DepositorInsertBtGH84
UseFor in vitro transcription from t3 promoter after…TagsSV40 Nuclear Localization Signal, SV40 Nuclear Lo…ExpressionMutationAspartic Acid 242 (in Dennis et al.) changed to A…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SLC38A9.1
Plasmid#71869PurposeoverexpressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseTagsHAExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin/Δβ-catenin-pcDNA3
Plasmid#45772DepositorInserthE-cadherin/Δβ-catenin (CDH1 Human)
UseTagsExpressionMammalianMutationEnds at amino acid 844. Last 35 amino acids dele…PromoterCMVAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag MKK6(K82A)
Plasmid#13519DepositorInsertMKK6(K82A) (MAP2K6 Human)
UseTagsFlagExpressionMammalianMutationLys-82AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
opto-Cdc42
Plasmid#174510PurposeExpresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.DepositorInsertopto-Cdc42-mCherry
UseTags3xFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-beta-catenin-IRES-GFP
Plasmid#14717DepositorInsertbeta-catenin (Ctnnb1 Mouse)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ mWnt5a
Plasmid#102860PurposeExpresses mouse Wnt5a in mammalian cells, zebrafish or XenopusDepositorInsertMouse Wnt5a
UseZebrafish expression; xenopus expressionTagsExpressionMammalianMutationPromoterCMV; SP6Available sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG6 CTF-FLAG
Plasmid#217704PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR6 (ADGRG6 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-840PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG
Plasmid#217737PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR6 (ADGRG6 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-846PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Renilla luciferase-Pol III
Plasmid#37380DepositorInsertPolIII-Renilla control reporter (RpIII128 Fly)
UseLuciferaseTagsExpressionMammalianMutationPromoterRNA PolIII 128 subunit (RpIII128)Available sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
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3XFLAG_ALDOA in pCW57_tTA_Blast
Plasmid#154210PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOADepositorInsertALDOA (ALDOA Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psRaf1
Plasmid#89364PurposeA lenti-viral vector expressing photoswitchable Raf1 in mammalian cellsDepositorInsertpsRaf1 (RAF1 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
Plasmid#173870PurposeMammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)DepositorInsertmCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
bArr2-ALFA
Plasmid#201498PurposeExpression of the beta-arrestin2 containing a N-terminal ALFA tag.DepositorInsertbArr2-ALFA (ARRB2 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT8-mGold
Plasmid#183989PurposeExpresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag8 (C61S, C262S), mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3.1-Flag-H2A K13-15R
Plasmid#63563PurposeExpression of human H2A with K13R and K15R tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysine 13 and Lysine 15 are now ArgininePromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-HBXIP
Plasmid#42326DepositorInsertHBXIP (LAMTOR5 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only
