Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 8,780 results for: construct

Clear keywords
Showing: 5001 - 5040 of 8780 results

  9. pDBD-M

    Plasmid
    #50572
    Purpose
    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Promoter
    Gal1
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pSMART-2,3-BDO1

    Plasmid
    #65816
    Purpose
    NADH Dependent 2,3-BDO Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    budA: α-acetolactate decarboxylase
    budB: acetolactate synthase
    budC: acetoin reductase
    Expression
    Bacterial
    Promoter
    waaHp from E. coli as part of operon with budA an…
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    65816-plasmid-map-sequence-id-111948

    Enlarge

  16. pPEE27

    Plasmid
    #128367
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 3, a small gene-free region. Contains the nourseothricin resistance marker (NAT)
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Promoter
    ACT1
    Available Since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128367-plasmid-map-sequence-id-254592

    Enlarge

  24. pAAV-Hsyn-Sun1sfGFP

    Plasmid
    #222510
    Purpose
    AAV packaging construct for expression Sun1-sfGFP fusion Protein under the human synapsin 1 gene promoter
    Depositor
    Sarkis Mazmanian
    Insert
    Sun1-sfGFP (Sun1 Mouse)
    Use
    AAV
    Available Since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    222510-plasmid-map-sequence-id-441103

    Enlarge

  25. pAAV-VTKS4-GCaMP6f

    Plasmid
    #209931
    Purpose
    Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter. Based on construct from Pouchelon et al Cell Rep Methods. 2022.
    Depositor
    Jessica Cardin
    Insert
    GCaMP6f
    Use
    AAV, CRISPR, and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    209931-plasmid-map-sequence-id-414074

    Enlarge

  28. pCR241

    Plasmid
    #240644
    Purpose
    Expression of AmNeonGreen-13myc in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    Use
    Gene expression and genomic integration in fish
    Tags
    13myc
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    240644-plasmid-map-sequence-id-482908

    Enlarge

  30. Rab11A[8-175]-His-C Q70L

    Plasmid
    #228042
    Purpose
    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).
    Depositor
    Wade Harper
    Insert
    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    Expression
    Bacterial
    Mutation
    Q70L
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228042-plasmid-map-sequence-id-450388

    Enlarge

  38. pMbd3EosHaloTAF_Hyg

    Plasmid
    #140958
    Purpose
    Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    methyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140958-plasmid-map-sequence-id-338424

    Enlarge

  39. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

    Enlarge

  40. pPEE36

    Plasmid
    #128376
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 7, a small gene-free region. Contains the NEO resistance marker
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Promoter
    ACT1
    Available Since
    April 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    128376-plasmid-map-sequence-id-292552

    Enlarge

Showing: 5001 - 5040 of 8780 results