We narrowed to 4,339 results for: 187

1. pEntry_HCoV-229E-nsp12

   Plasmid

   #168969

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-229E-nsp12 (HCoV229Egp1 )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAFMW-Nbr-D435A/E437A

   Plasmid

   #40829

   Purpose

   Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

   Depositor

   Phillip Zamore

   Article

   Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

   Insert

   Nbr D435A/E437A (Nbr Fly)
   

   Use

   Tags

   3xFLAG and 6xMyc

   Expression

   Insect

   Mutation

   D435A/E437A, part of the DEDD motif; mutations p…

   Promoter

   Actin5C

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-hDcp2 E147Q, E148Q

   Plasmid

   #72215

   Purpose

   bacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E147Q, E148Q

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pHH0103_PRKACA_C1+C2

   Plasmid

   #110027

   Purpose

   Protein expression and purification of PRKACA_C1+C2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PRKACA_C1+C2 (PRKACA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. b2-TS3

   Plasmid

   #103877

   Purpose

   full-length human b2 with tension sensor at b2_733

   Depositor

   Timothy Springer

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   full-length human b2 with tension sensor at b2_733 (ITGB2 Human)
   

   Use

   Tags

   tension sensor module

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSG5-Myr-FLAG-p110α-DM

   Plasmid

   #55713

   Purpose

   eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Use

   Tags

   myristoylation tag (Myr-FLAG)

   Expression

   Mammalian

   Mutation

   T207D, K227A

   Promoter

   T7

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pEntry_HCoV-229E-nsp8

   Plasmid

   #168957

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-229E-nsp8 (HCoV229Egp1 )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NUDT16

   Plasmid

   #155794

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   NUDT16 (NUDT16 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JBEI-3716

   Plasmid

   #100169

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   codon-optimized MK
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV FLIP p53

    Plasmid

    #19745

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCerulean3-Alpha-5-Integrin-12

    Plasmid

    #55401

    Purpose

    Localization: Focal Adhesions, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Alpha-5 Integrin (ITGA5 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pEntry_HCoV-229E-nsp5

    Plasmid

    #168954

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-nsp5 (HCoV229Egp1 )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mutpRL-TK 3xUTR

    Plasmid

    #40758

    Purpose

    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

    Depositor

    Phillip Zamore

    Article

    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

    Insert

    3 copies of the CXCR4 small RNA target site
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    mutated from TAG to CTC at nt 387–389 of the Reni…

    Promoter

    TK

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pAcGP67A-hBAI3-HormR-GAIN

    Plasmid

    #37848

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    BAI3 (BAI3 Human)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Expression

    Mutation

    HormR and GAIN domains only (aa498–868

    Promoter

    Polyhedrin

    Available since

    July 6, 2012

    Availability

    Academic Institutions and Nonprofits only

15. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PKM-IRES-hrGFP

    Plasmid

    #73405

    Purpose

    Mammalian expression of mouse PKM and hrGFP

    Depositor

    Raymond Swanson

    Article

    Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

    Insert

    PKM (Prkcz Mouse)
    

    Use

    Tags

    3xFLAG and pIRES-hrGFP-2a

    Expression

    Mammalian

    Mutation

    truncated, constitutively active form

    Promoter

    CMV

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. EGFP-UVRAG S498A

    Plasmid

    #86741

    Purpose

    N-terminal EGFP-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S498A

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-UVRAG S498A

    Plasmid

    #86744

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S498A

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAT-mWasabi-G

    Plasmid

    #164170

    Purpose

    Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPBpuro-mNG-eDHFR(69K6)-p85iSH2

    Plasmid

    #172103

    Purpose

    Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge