We narrowed to 17,154 results for: REV

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pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAG303GPD-ccdB

Plasmid

#14134

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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SLC25A1_pLX307

Plasmid

#98374

Purpose

Lentiviral expression of SLC25A1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

SLC25A1 (SLC25A1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pHR-mCherry-tSH2(ZAP70)

Plasmid

#27137

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

mCherry and ZAP70 (residues 1-259) (ZAP70 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA-BfuAI-2k

Plasmid

#112915

Purpose

Empty sgRNA expression plasmid

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-EGFP-ccdB

Plasmid

#14322

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Yeast

Available Since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pNeonGreen-C1-Nir1-LNS2

Plasmid

#220083

Purpose

High affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM3 (PITPNM3 Human)

Tags

NeonGreen-GGSGGM

Expression

Mammalian

Mutation

amino acids 613-897

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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Profiline

Plasmid

#89925

Purpose

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

Depositor

Antoine Jégou, Guillaume Romet-Lemonne

Article

Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

Insert

profiline-1 (PFN1 Human)

Tags

None

Expression

Bacterial

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomEos2-Kan

Plasmid

#44944

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Expression

Yeast

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-ZIP(WT)

Plasmid

#27134

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) NAPstar3b

Plasmid

#232736

Purpose

Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar3b

Expression

Mammalian

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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RGB-S reporter

Plasmid

#207841

Purpose

A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

Depositor

Christof Niemeyer

Article

Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

Inserts

RpoH sensing construct
SOS sensing construct
RpoS sensing construct

Expression

Bacterial

Promoter

PosmY, PsulA, and grpE

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAuroraA-GFP-AURKA-mCherry

Plasmid

#99878

Purpose

Expression of GFP-AURKA-mCherry in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP and mCherry

Expression

Mammalian

Promoter

AURKA minimal

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-sox10-NLS-BirA-2A-mCherry_Ras

Plasmid

#80063

Purpose

Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Sox10 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBa-flag-BicD2 594-FKBP

Plasmid

#64206

Purpose

Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pX330 _CRISPIE donor B7

Plasmid

#172848

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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HA-GABARAPL2

Plasmid

#137761

Purpose

Expression of GABARAPL2

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL2 (GABARAPL2 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v1

Plasmid

#97081

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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