We narrowed to 6,546 results for: proc

1. pLIC-myc-GBAi W211A

   Plasmid

   #171754

   Purpose

   For the mammalian expression of myc-GBAi W211A

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Use

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CAG-FLEX-BFP2-(mCherry)’

   Plasmid

   #115234

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNCX2-mEGFP-VPS4A

   Plasmid

   #116924

   Purpose

   expresses mEGFP-VPS4A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   silent mutation in position 4059 C to T

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. En1

   Plasmid

   #34993

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Homeobox protein engrailed-1 (En1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKO3-TEAD4

   Plasmid

   #221217

   Purpose

   RFP fused TEAD4

   Depositor

   Xiaokun Shu

   Article

   Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

   Insert

   mKO3-TEAD4 (TEAD4 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDACR3882 [rab-3p::HYlight::let-858utr]

   Plasmid

   #207666

   Purpose

   Expresses worm-optimized HYlight in C. elegans neurons

   Depositor

   Daniel Colón-Ramos

   Article

   Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   HYlight Biosensor
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. retro-gRNA-eGFP

   Plasmid

   #116926

   Purpose

   Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

   Depositor

   Diane Mathis, Christophe Benoist

   Article

   Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Tubulin-C-18

   Plasmid

   #55148

   Purpose

   Localization: Microtubules, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

    Plasmid

    #187113

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSG00000101199 (ARFGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-UTX-F

    Plasmid

    #17438

    Purpose

    Expression of UTX in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    UTX (KDM6A Human)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nef-CIAO2-Flp

    Plasmid

    #149296

    Purpose

    Cre-dependent expression of Flp recombinase.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    Flp recombinase (flp Budding Yeast)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Derived from the mouse codon-optomized FlpO. The…

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-mGreenLantern

    Plasmid

    #161913

    Purpose

    mGreenLantern fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag hHMGB1

    Plasmid

    #31609

    Depositor

    Yasuhiko Kawakami

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCasTet-λ

    Plasmid

    #128318

    Purpose

    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

    Depositor

    Christopher Johnston

    Article

    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

    Insert

    TetR and pTetR/TetO
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Constitutive expression of cas9 and anhydro-tetra…

    Promoter

    tet promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52544

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4-Beclin1 (FL)

    Plasmid

    #24388

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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