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pEGFP-SF2

Plasmid

#17990

Depositor

Tom Misteli

Article

Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

Insert

SF2 (SRSF1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains SF2 (aa1-210)

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-UBF

Plasmid

#17656

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

UBF (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 28, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV5 mouse src

Plasmid

#13663

Depositor

Joan Brugge, Peter Howley

Insert

src (Src Mouse)

Tags

Myr

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6 ActQ67L

Plasmid

#10835

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 Q67L (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L. Defective in GTP hydrolysis. Constitutive…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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HA.PKD

Plasmid

#10808

Depositor

Alex Toker

Article

Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Insert

PKD (PRKD1 Human)

Tags

HA

Expression

Mammalian

Mutation

L520P

Promoter

CMV

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6b

Plasmid

#207852

Purpose

Mammalian expression of PE6b prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6b

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide Puro-P2A-EGFP

Plasmid

#137729

Purpose

For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

Depositor

Fredrik Wermeling

Article

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2 (GFP)

Plasmid

#80924

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA GNSTM-3-RVG-10-Lamp2b-HA

Plasmid

#71294

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

GNSTM glycosylation motif, HA, Lamp2 signal pepti…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt1

Plasmid

#66830

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLH-spsgRNA2

Plasmid

#64114

Purpose

Vector for expression of Sp sgRNA2 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp sgRNA2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XmCherry

Plasmid

#64110

Purpose

Expression of Nm dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-Cetn1/1

Plasmid

#50718

Purpose

pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Inserts

Cetn1 sgRNA1
humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh and hU6

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-ASC-HA

Plasmid

#41553

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

ASC (PYCARD Human)

Tags

HA

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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