165,307 results

1. Anti-6xHis [N144/14R]

   Plasmid

   #177500

   Purpose

   Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-6xHis recombinant mouse monoclonal antibody
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-f:PGC1

   Plasmid

   #1026

   Depositor

   Bruce Spiegelman

   Article

   Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

   Insert

   PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTIPI_mIGg2a_Anti-HA [12CA5]

   Plasmid

   #210656

   Purpose

   Mammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Anti-HA [12CA5] mouse IgG2a heavy chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Mouse IgG2a heavy chain

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTIPI_mK_Anti-HA [12CA5]

   Plasmid

   #210657

   Purpose

   Mammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Anti-HA [12CA5] kappa light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Mouse kappa light chain

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFUGW-FerH-ffLuc2-eGFP

   Plasmid

   #71393

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   FerH-ffLuc2-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   FerH

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AYBEv3

   Plasmid

   #193967

   Purpose

   An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(v3)
   

   Expression

   Mammalian

   Mutation

   G163R, N169S, S198A, K202A, G203A, S206A, K210A

   Promoter

   hU6, EF1a, CBh

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27-Tornado-RhoBAST-MS2

   Plasmid

   #196342

   Purpose

   Overexpression of circular RhoBAST-MS2

   Depositor

   Murat Sunbul

   Article

   Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

   Insert

   Tornado-RhoBAST-MS2
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-GATA4

   Plasmid

   #192896

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Codon-optimized

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

   Plasmid

   #175529

   Purpose

   Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag7
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN-I-GFP

    Plasmid

    #1736

    Purpose

    This retroviral vector has an IRES-GFP downstream of the cloning sites

    Depositor

    Garry Nolan

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

    Plasmid

    #156440

    Purpose

    For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA1 (Stag1 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

    Plasmid

    #31484

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    EF1s HA NLS Sce(opt) T2A IFP1.4
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    "Opt" indicates the I-SceI coding seque…

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMito-MtrxRFP2

    Plasmid

    #172324

    Purpose

    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPAmCherry-a-tubulin

    Plasmid

    #31930

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Tags

    a-tubulin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-wtFAKSPARK2

    Plasmid

    #195263

    Purpose

    express wtFAKSPARK2

    Depositor

    Xiaokun Shu

    Article

    Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

    Insert

    FAKSPARK2 sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196084

    Purpose

    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLNHA-C1-HsXRN1

    Plasmid

    #66596

    Purpose

    expresses lambdaN-HA-tagged XRN1 in mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    XRN1 (XRN1 Human)
    

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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