4,176 results

1. pSRK2706

   Plasmid

   #78571

   Purpose

   Expresses untagged human rhinovirus 3C protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

   Insert

   human rhinovirus 3C protease (HRVBgp1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-RFP-P2A-MCS

   Plasmid

   #78933

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-jGCaMP7s

   Plasmid

   #104463

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7s
   

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3 Flag p38 alpha

   Plasmid

   #20351

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 alpha (Mapk14 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+) Drp1

   Plasmid

   #34706

   Depositor

   David Chan

   Insert

   Drp1 (Dnm1l Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-ATG5-K130R

   Plasmid

   #13096

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5-K130R (Atg5 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   K130R

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1136- Desmoplakin-GFP

   Plasmid

   #32227

   Depositor

   Kathleen Green

   Article

   Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

   Insert

   Desmoplakin (DSP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

   Plasmid

   #50458

   Purpose

   Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV8

   Insert

   rM3D-mCherry
   

   Use

   AAV

   Tags

   HA and mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ1661-sgMUC4-E3(F+E)

   Plasmid

   #51025

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   optimized sgRNA (MUC4 Human, Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-AKT-PH-GFP

    Plasmid

    #18836

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    AKT-PH domain (AKT1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1-148 (compared to GenBank r…

    Promoter

    CMV

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo beta catenin S33Y

    Plasmid

    #16519

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    beta catenin (CTNNB1 Human)
    

    Expression

    Mammalian

    Mutation

    S33Y (SW48 mutation)

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSin Capsid

    Plasmid

    #127693

    Purpose

    Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Bryan Roth, Justin English

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Capsid
    

    Use

    Synthetic Biology

    Available Since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid

    #102245

    Purpose

    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

    Depositor

    Jacob Corn

    Insert

    dCas9-VPR-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-VPR-mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgRNA-modified

    Plasmid

    #84477

    Purpose

    guide RNA expression lentiviral construct

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    guide RNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-Ikkb-FLAG WT

    Plasmid

    #23298

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    Ikkbeta (IKBKB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-Chk1-Flag

    Plasmid

    #22894

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Chk1 (CHEK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-C1-CAMKIIalpha

    Plasmid

    #21226

    Depositor

    Tobias Meyer

    Article

    Shen et al Neuron. 1998 Sep . 21(3):593-606.

    Insert

    CAMKIIalpha (Camk2a Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lab plasmid KS 0001.

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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