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pCMV-HA-mIFITM1-C83A

Plasmid

#58418

Purpose

Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 83 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C50A

Plasmid

#58417

Purpose

Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 50 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49A

Plasmid

#58416

Purpose

Expresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Cysteine 49 to Alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 M2 (Y115A, Y148A, Y251A)

Plasmid

#44030

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

Y115A Y148A Y251A

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
pTH729-minRLuc

Plasmid

#38225

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Mutation

Every codon of the Renilla coding sequence was ex…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTH738-CENBEVYslow

Plasmid

#38220

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

GCN4-based single uORF-containing 5'-UTR

Expression

Yeast

Mutation

The start codons of uORFs2, 3 and4 of the yeast G…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pHL1324

Plasmid

#37638

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pCon and pConNoHind

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
PCDNA3 T7 DLC2

Plasmid

#24268

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

T7

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGSTag BimEL (3SA)

Plasmid

#23100

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (3SA) (Bcl2l11 Mouse)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 55/65/73 to alanine

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A-H195A

Plasmid

#20236

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Mutation

changed His 195 to Ala

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2A

Plasmid

#24180

Purpose

Mammalian expression of human JMJD2A with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2A (KDM4A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available Since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

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pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

Plasmid

#84258

Purpose

Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

Sp sgTRE3G
PYL1-VPR

Use

CRISPR and Lentiviral

Tags

IRES-mCherry and PYL1

Expression

Mammalian

Promoter

CMV and mouse U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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