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B*58:02:01

Plasmid

#203262

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*58:02:01 (HLA-B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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A*66:01:01

Plasmid

#203291

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*66:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194374

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-NTEV-TCS-GV-2xHA (GCGR Human, Synthetic)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBC2T-lamin

Plasmid

#108972

Purpose

expresses BC2-tag-lamin in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

lamin B1 (LMNB1 Human)

Use

Tags

BC2-tag

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DYNLL1_Dynein-light

Plasmid

#110006

Purpose

Protein expression and purification of DYNLL1_Dynein-light

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DYNLL1_Dynein-light (DYNLL1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pEYFP_C1

Plasmid

#85766

Purpose

mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

catalytic domain (aa 212-514)

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3_DRD1-NTEV-TCS-GV-2xHA

Plasmid

#194358

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD1-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI2_G-alpha

Plasmid

#109791

Purpose

Protein expression and purification of GNAI2_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI2_G-alpha (GNAI2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_VCP_AAA

Plasmid

#109964

Purpose

Protein expression and purification of VCP_AAA

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCP_AAA (VCP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-FGF21

Plasmid

#182407

Purpose

Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-fgf21 (FGF21 Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLCG1_SH3

Plasmid

#110035

Purpose

Protein expression and purification of PLCG1_SH3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLCG1_SH3 (PLCG1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNA11_G-alpha

Plasmid

#109911

Purpose

Protein expression and purification of GNA11_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA11_G-alpha (GNA11 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD4S

Plasmid

#197882

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_AURKB_Pkinase

Plasmid

#110025

Purpose

Protein expression and purification of AURKB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AURKB_Pkinase (AURKB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAav-MCS-PQS2-3xHA

Plasmid

#84917

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

LOX-PGK-NEO-LOX

Use

AAV

Tags

PQS2 3xHA

Expression

Mutation

Promoter

mPGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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A*01:03:01

Plasmid

#203293

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*01:03:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-HIS-ddFLN4-K10

Plasmid

#101718

Purpose

K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)

Use

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEBG-IFNAR2(265-515)

Plasmid

#12437

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

IFNAR2 a.a. 265-515 (IFNAR2 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
VIPR1

Plasmid

#51865

Purpose

Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

VIPR1 (VIPR1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_AKT1_Pkinase

Plasmid

#109775

Purpose

Protein expression and purification of AKT1_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AKT1_Pkinase (AKT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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