We narrowed to 3,078 results for: cat.3

1. pAc-sgRNA-Cas9.dora_3

   Plasmid

   #190702

   Purpose

   Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   dora (CG34401) sgRNA 3
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila U6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBO.ACT004

   Plasmid

   #182714

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1

   Plasmid

   #193356

   Purpose

   VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 (KLF4, SOX2, POU5F1 Human)
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CXCR4-SZ158a QTY

   Plasmid

   #162442

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-X-C chemokine receptor type 4 (CXCR4 Human)
   

   Use

   Tags

   his-tag, Rho-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. CXCR4-SZ146a QTY

   Plasmid

   #162443

   Purpose

   Expression in E coli and perform ligand binding in solution

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-X-C chemokine receptor type 4 (CXCR4 Human)
   

   Use

   Tags

   his-tag, Rho-tag

   Expression

   Bacterial

   Mutation

   All L, I, V, F in the transmembrane region mutate…

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. CXCR4-SZ158a-sfGFP

   Plasmid

   #162446

   Purpose

   Expression in HEK293T cell and compete ligand singaling against full-length receptors

   Depositor

   Shuguang Zhang

   Article

   Qing et al iScience. 2020 Oct 28;23(12):101670. doi

   Insert

   C-X-C chemokine receptor type 4 (CXCR4 Human)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   truncation from aa52 to aa257

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hsyn-ASAP5-WPRE

   Plasmid

   #225709

   Purpose

   Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Insert

   ASAP5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human synapsin

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-ASAP5-Kv2.1-WPRE

   Plasmid

   #225707

   Purpose

   Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human synapsin

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

    Plasmid

    #225708

    Purpose

    Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-shRNA-Tet1

    Plasmid

    #85742

    Purpose

    shRNA against mouse Tet1

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFusX1-20

    Plasmid

    #63238

    Purpose

    Encodes RVD positions 1-3 in TALEN assembly

    Depositor

    Stephen Ekker

    Article

    Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

    Insert

    HD NI NG
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-EF1a-DIO-ASAP3-WPRE

    Plasmid

    #132318

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-hDAGLa-V5

    Plasmid

    #87674

    Purpose

    Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Article

    Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do

    Insert

    Human Diacylglycerol Lipase Alpha (DAGLA Human)
    

    Use

    Tags

    HA and V5 tag

    Expression

    Mammalian

    Mutation

    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

    Promoter

    T7

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSrtA(4S-9) pET29b

    Plasmid

    #75146

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N98D S102C A104V A118T F122A K134R F144L I182V E1…

    Promoter

    T7

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TadCBE-Cas9NG

    Plasmid

    #225093

    Purpose

    Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

    Insert

    TadCBE-Cas9NG
    

    Use

    CRISPR

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Col2.3deltaTK

    Plasmid

    #114273

    Purpose

    Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

    Depositor

    David Rowe

    Article

    Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

    Insert

    Col2.3 promoter + deltaTK + BGH polyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2.3 kb rat type 1 collagen

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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