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CMV-NF186(K519TAG)-HA

Plasmid

#239775

Purpose

Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K519TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K534TAG)-HA

Plasmid

#239776

Purpose

Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K534TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K571TAG)-HA

Plasmid

#239777

Purpose

Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K571TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K604TAG)-HA

Plasmid

#239778

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K680TAG)-HA

Plasmid

#239779

Purpose

Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K680TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K809TAG)-HA

Plasmid

#239780

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

Plasmid

#235591

Purpose

Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dot1l (Dot1l Mouse)

Use

CRISPR

Mutation

GS163-164RC

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015869

Plasmid

#226281

Purpose

Expresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

Depositor

Rainer Breitling

Article

Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

Inserts

4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSCrhaB2_ApyOAHIDS

Plasmid

#226246

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyOAHID

Plasmid

#226245

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGM9

Plasmid

#220167

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

FUNDC1 (FUNDC1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G137I)

Plasmid

#133272

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Use

Oocyte expression

Mutation

G137I

Promoter

T7

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Amber-free HIV-1 deltaRT

Plasmid

#213007

Purpose

An replication-incompetent amber-free HIV-1 encodes full-length Env BG505 with RT-deleted Q23 as the backbone.

Depositor

Maolin Lu

Article

Ao et al Cell Chem Biol. 2024 Jan 8:S2451-9456(23

Inserts

HIV-1 Q23 ΔRT backbone (reverse transcriptase is deleted)
Env BG505

Use

Unspecified

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

Plasmid

#189632

Purpose

Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 hU6 ACTB sgRNA

Plasmid

#206270

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

ACTB sgRNA

Use

CRISPR; Multimate/gateway entr 2

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 hU6 HEK3 PegRNA

Plasmid

#206277

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

HEK3 PegRNA

Use

CRISPR; Multimate/gateway entr 2

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 3 polH aeBlue polH VSV-G

Plasmid

#206278

Purpose

ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

aeBlue, VSV-G

Use

Multimate/gateway entr 3

Expression

Bacterial and Insect

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT_-100_-79

Plasmid

#193785

Purpose

luciferase reporter for duplicated TERT promoter

Depositor

Joseph Costello

Article

Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

Insert

TERT promoter (TERT Human)

Use

Luciferase

Expression

Mammalian

Promoter

TERT

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDA2ScIfm

Plasmid

#193786

Purpose

Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

Frame-shifted CI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTATAA synthetic promoter carrying binding sites…

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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