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YFP-DAT

Plasmid

#90228

Purpose

Expresses human DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAF0084 Ef1a-Cp36-2a-EGFP

Plasmid

#193462

Purpose

Expression of Cp36 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Plasmid

#175777

Purpose

pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TMEM192 (TMEM192 Human)

Use

CRISPR

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Expression

Yeast

Promoter

ADH1

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJW2171

Plasmid

#163095

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-CAAX-SV40

Plasmid

#117809

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Desmoglein1-N-18

Plasmid

#55030

Purpose

Localization: Desmosomes, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Desmoglein1 (DSG1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CCL21-V5

Plasmid

#11609

Depositor

Steven Johnson

Insert

chemokine (C-C motif) ligand 21 (CCL21 Human)

Tags

V5

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

Plasmid

#128607

Purpose

Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

36xMashTag

Expression

Mammalian

Promoter

Pcmv

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pOT_2 - lenti-EFS-tNGFR-2A-puro

Plasmid

#181971

Purpose

Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

tNGFR (NGFR Human)

Use

Lentiviral

Mutation

truncation: aa 11-276

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma13-GFP2

Plasmid

#140992

Purpose

Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma13-GFP2 (GNG13 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#121151

Purpose

Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K6 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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tCRMod-GGSGGS9

Plasmid

#111762

Purpose

Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-GGSGGS9-mRuby2 fusion

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDECKO_Malat1_C

Plasmid

#72622

Purpose

Expresses two gRNAs targeting MALAT1 promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward Malat1

Expression

Mammalian

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-off TauRD (P301L/V337M)

Plasmid

#188572

Purpose

Dox-repressible expression of the Tau repeat domain

Depositor

Franz-Ulrich Hartl

Article

Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

Insert

Tau repeat domain (MAPT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

P301L and V337M

Promoter

pTight TRE

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

Plasmid

#187588

Purpose

Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NLS

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRMT1-Halo

Plasmid

#175334

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRMT1 (PRMT1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Dvl2-GFP

Plasmid

#109148

Purpose

Stable Dvl2 expression

Depositor

Mariann Bienz

Article

Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

Insert

Dvl2 (DVL2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

SV40

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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