We narrowed to 32,430 results for: uros

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pLVX-TetOne-Puro-EcSTH-FLAG

Plasmid

#218628

Purpose

This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

EcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TLCV2-Scal-T2A-EGFP-Puro

Plasmid

#234949

Purpose

Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2-ApaLI-T2A-EGFP-Puro

Plasmid

#234948

Purpose

Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mAscl1

Plasmid

#198755

Purpose

Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Promoter

TRE3G

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW PuroR-P2A-COX8ASig-Timer

Plasmid

#239559

Purpose

Expression and mitohcondrial localization of Timer

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Timer

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Myr-Flag-AKT1

Plasmid

#15294

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-HA-FLAG-AU1

Plasmid

#162118

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to HA-FLAG-AU1

Use

Lentiviral

Tags

HA-FLAG-AU1

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO eGFP Puro (w159-1)

Plasmid

#17481

Purpose

3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV PIG (Puro IRES GFP)

Plasmid

#18751

Depositor

Scott Lowe

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pRCA360 - pBA904 Puro-T2A-GFP

Plasmid

#238164

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-Puro SPI1 P2A mCherry

Plasmid

#140029

Purpose

Tunable and temporal expression control of SPI1 and mCherry

Depositor

Xiaoping Bao

Insert

SPI1 (SPI1 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

TRE3G

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-mitoEcSTH-FLAG

Plasmid

#218629

Purpose

This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

mitoEcSTH

Use

Lentiviral and Synthetic Biology

Tags

FLAG and MTS

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-EEA-5guides-PGK-Puro

Plasmid

#102898

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5guides-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-GFP-miRE_shRen-Puro

Plasmid

#135683

Purpose

Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

Depositor

Florian Karreth

Insert

shRenilla

Use

Lentiviral

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR1-SV40-puro

Plasmid

#240535

Purpose

Lentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-tdTomato-miRE_shRen-Puro

Plasmid

#135687

Purpose

Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

Depositor

Florian Karreth

Insert

shRenilla

Use

Lentiviral

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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