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Showing: 361 - 390 of 1192 results

  1. pRegev_D1

    Plasmid
    #204178
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D1 in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204178-plasmid-map-sequence-id-405445

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  3. pRegev_S5.49C>A

    Plasmid
    #204175
    Purpose
    pRegev_S5 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S5 with 49C>A mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    49C>A mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204175-plasmid-map-sequence-id-405426

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  4. pRegev_D4

    Plasmid
    #204184
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    D4 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204184-plasmid-map-sequence-id-405407

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  5. pRegev_D2

    Plasmid
    #204180
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D2 in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    61C>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204180-plasmid-map-sequence-id-405406

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  7. pRegev_ExonSkip_Backbone

    Plasmid
    #204187
    Purpose
    Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    First exon and intron for beta globin splicing minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    204187-plasmid-map-sequence-id-404040

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  9. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154846-plasmid-map-sequence-id-348286

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  16. EK0379 CMV-TO-EGFP-stop-t70587 (PB)

    Plasmid
    #191154
    Purpose
    Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    EGFP-t70587
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV-TO
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191154-plasmid-map-sequence-id-383934

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  17. pS148∙CsR

    Plasmid
    #197858
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Article
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Insert
    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197858-plasmid-map-sequence-id-393255

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  18. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  19. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid
    #191164
    Purpose
    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP (additional cutsites)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191164-plasmid-map-sequence-id-375473

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  22. 908H

    Plasmid
    #160240
    Purpose
    TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Omar Akbari
    Article
    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.
    Inserts
    Tet Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Use
    Tags
    T2A GFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160240-plasmid-map-sequence-id-316017

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  23. p5M-hPR-B

    Plasmid
    #89114
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 restriction sites deleted in polylinker
    Promoter
    CMV
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89114-plasmid-map-sequence-id-267772

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Showing: 361 - 390 of 1192 results