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Plasmid#236236PurposeAAV expression of human STIM2 internally tagged with mScarlet-IDepositorInsertmScarletI-STIM2 (STIM2 Human)
UseAAVTagsmScarletI in position 29ExpressionMutationSignal peptide from STIM1 and residues 15-746 of …Promoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mEmerald-STIM1 WPRE
Plasmid#236234PurposeAAV expression of mouse STIM1 internally tagged with mEmeraldDepositorInsertmEmerald-Stim1 (Stim1 Mouse)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-STIM2 WPRE
Plasmid#236235PurposeAAV expression of human STIM2 internally tagged with HaloTagDepositorInsertHaloTag-STIM2 (STIM2 Human)
UseAAVTagsHaloTag in position 29ExpressionMutationSignal peptide from STIM1 and residues 15-746 of …Promoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mouse STING #1-gRNA-Cas9-Puro
Plasmid#186892PurposegRNA targeting mouse STING (with Cas9 insert)DepositorInsertCas9 (Sting1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mouse STING #2-gRNA-Cas9-Puro
Plasmid#186893PurposegRNA targeting mouse STING (with Cas9 insert)DepositorInsertCas9 (Sting1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-STING L373A with IRSp53 pLxIS
Plasmid#221283PurposeExpression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , F124D, residues 108-200
Plasmid#180629PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200, F124DPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 SPASTIN , L177D, residues 108-200
Plasmid#180634PurposeBacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 108-200, L177DPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D
Plasmid#198765Purposeexpression of hOCT4 mutantsDepositorInserthOCT4 236D (POU5F1 Human)
UseTagsFLAGExpressionMammalianMutationS236DPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-uTEV1-V5; UAS-mCherry
Plasmid#173122PurposeExpresses uTEV1 and reporter receiver construct in DIPGDepositorInsertArrestin-uTEV1-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry
Plasmid#173121PurposeExpresses TEVp(WT) and reporter receiver construct in DIPGDepositorInsertArrestin-TEVp(WT)-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target
Plasmid#159842PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertpDESTR4-R3 mosTI Pmlc-2_GFP target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM322 - array co-injection fragment mosTI neoR
Plasmid#159832PurposeSingle copy or array insertion by MosTI using split NeoR selectionDepositorInsertarray co-injection fragment mosTI neoR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG172-STII PLU-HIS
Plasmid#112992PurposeBaculovirus expression of Drosophila png172-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationpng172 mutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-STII PLU-HIS
Plasmid#112993PurposeBaculovirus expression of Drosophila png-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)
Plasmid#107247Purposebeta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.DepositorArticleInsertARRB2
UseAAVTagsMYC, PYLcs, and YFPExpressionMutationPromoterhSYNAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEM318 - sgRNA mosTI unc-119 single-copy
Plasmid#159822PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertsgRNA mosTI unc-119 single-copy
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM319 - array co-injection fragment mosTI unc-119
Plasmid#159823PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertarray co-injection fragment mosTI unc-119
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin
Plasmid#187956PurposeFKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-hHDAC4-SpdCas9-tagBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro
Plasmid#216247PurposeExpress OsTIR1.F74A mutant with mCherry tag for AID2DepositorInsertOsTIR1.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin
Plasmid#187953PurposeFKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-SpdCas9-KRAB-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK Lentivirus Multi Cassette Destination Vector (pAN2424)
Plasmid#194221PurposeBackbone for constructing multi casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125228PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM320 - sgRNA mosTI unc-119 array insertion
Plasmid#159824PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertsgRNA mosTI unc-119 array insertion
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2
Plasmid#182348PurposemScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2
Plasmid#182352PurposePan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin
Plasmid#187957PurposeFKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-KRAB-SpdCas9-tagBFP
UseCRISPR and Synthetic BiologyTagsGGSExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-YFP-Rb-IRES-blasticidin
Plasmid#223962PurposeDual fluorescent reporter for histone H2B and for exogenous RbDepositorUseTagsYFP and iRFP670ExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTK Lentivirus Single Cassette Destination Vector (pAN2414)
Plasmid#194220PurposeBackbone for constructing single casette expression plasmids using the Mammalian TookitDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only