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We narrowed to 171,429 results for: addgene

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Showing: 481 - 520 of 171429 results

  1. pCAMTA2.1.0-gDNA

    Plasmid
    #132456
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CAMTA2 (CAMTA2 Human)
    Use
    CRISPR
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132456-plasmid-map-sequence-id-266911

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  2. pMBD1.1.0-gDNA

    Plasmid
    #132444
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    MBD1 (MBD1 Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132444-plasmid-map-sequence-id-266804

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  3. 6NIM

    Plasmid
    #127294
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD18
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 26, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    127294-plasmid-map-sequence-id-247944

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  4. 5VS7 (PP-BRD4)

    Plasmid
    #101264
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD4
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    101264-plasmid-map-sequence-id-193497

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  5. 5KO4 (PP-BRD19)

    Plasmid
    #101882
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD19
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101882-plasmid-map-sequence-id-194517

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  6. 5TCK (PP-BRD3)

    Plasmid
    #101887
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101887-plasmid-map-sequence-id-194702

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  7. 5U9N (PP-BRD3)

    Plasmid
    #101255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101255-plasmid-map-sequence-id-197534

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  8. 4QNY

    Plasmid
    #61958
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_331470.1:MAC04E-A10:C228347
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. PP-MAPK2 (3N9X)

    Plasmid
    #28117
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PBG-PF11_0147
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. PP-CDPK3

    Plasmid
    #28151
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDPK3
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    CAD domain (residues 425-602).
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid
    #232929
    Purpose
    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    OsTir1(F74G)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    changed Phenylalanine 74 to Glycine
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232929-plasmid-map-sequence-id-466497

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  15. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  17. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  19. ShuttleMCP

    Plasmid
    #50959
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to pShuttle-CMV (Addgene #16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter
    Depositor
    James Bamburg
    Article
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Promoter
    uses portion of the mouse cofilin gene as a mouse…
    Available Since
    Feb. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    50959-plasmid-map-sequence-id-76720

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  24. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  26. 5KKP (PUS7)

    Plasmid
    #101878
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PUS7 (PUS7 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101878-plasmid-map-sequence-id-194704

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  27. lenti-EF1a-dCas9-KRAB-MeCP2-Puro

    Plasmid
    #242163
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with puromycin reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-puro
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242163-plasmid-map-sequence-id-496076

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  28. pCMV_GFP_24

    Plasmid
    #238190
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP and FTH1 (24-mer), under a CMV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-FTH1 and SV40
    Expression
    Mammalian
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238190-plasmid-map-sequence-id-472656

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  30. lenti-EF1a-dCas9-KRAB-MeCP2-H2B-GFP

    Plasmid
    #242170
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242170-plasmid-map-sequence-id-496072

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  31. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-482202

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  34. pCMV-SUN1 mScarlet

    Plasmid
    #187683
    Purpose
    In vivo visualization of SUN1
    Depositor
    Mary Beckerle
    Insert
    SUN1 (Sun1 Mouse)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187683-plasmid-map-sequence-id-371441

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  35. pCB98

    Plasmid
    #69744
    Purpose
    Retroviral expression of Centrin 1
    Depositor
    Iain Cheeseman
    Insert
    Centrin 1 (CETN1 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Available Since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid
    #216668
    Purpose
    Expresses membrane-bound mCherry in mammalian cells
    Depositor
    Leonardo Morsut
    Insert
    mCherry
    Tags
    PDGFR Transmembrane Domain
    Expression
    Mammalian
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216668-plasmid-map-sequence-id-432187

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  37. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140082-plasmid-map-sequence-id-292704

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  38. pX552-GFP

    Plasmid
    #107023
    Purpose
    pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.
    Depositor
    Alex Hewitt
    Insert
    GFP
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107023-plasmid-map-sequence-id-208521

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Showing: 481 - 520 of 171429 results