We narrowed to 30,447 results for: Ide

1. pEGFP-(RTRG)2

   Plasmid

   #69846

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   2 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-(RTRG)3

   Plasmid

   #69847

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   3 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP-(RTRG)4

   Plasmid

   #69848

   Purpose

   The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.

   Insert

   4 RTRG repeats
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBGSA FPR1 R123A

   Plasmid

   #66721

   Purpose

   Constitutive expression of FPR1 R123A in mammalian cells.

   Depositor

   Heini Miettinen

   Article

   Miettinen et al J Biol Chem. 1999 Sep 24;274(39):27934-4

   Insert

   FPR1 (haplotype 3) (FPR1 Human)
   

   Expression

   Mammalian

   Mutation

   R123A

   Promoter

   SR-alpha

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pBGSA FPR1 D71A

   Plasmid

   #66720

   Purpose

   Constitutive expression of FPR1 D71A in mammalian cells.

   Depositor

   Heini Miettinen

   Article

   Miettinen et al J Biol Chem. 1999 Sep 24;274(39):27934-4

   Insert

   FPR1 (haplotype 3) (FPR1 Human)
   

   Expression

   Mammalian

   Mutation

   D71A

   Promoter

   SR-alpha

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pBGSA FPR1 H4 (haplotype 4)

   Plasmid

   #66718

   Purpose

   Constitutive expression of FPR1 in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Gripentrog et al Immunogenetics. 2008 Feb;60(2):83-93. do

   Insert

   FPR1 (haplotype 4) (FPR1 Human)
   

   Expression

   Mammalian

   Promoter

   SR-alpha

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

   Plasmid

   #117981

   Purpose

   SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B1
   

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

   Plasmid

   #117982

   Purpose

   SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B2
   

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA-humanized

   Plasmid

   #44248

   Purpose

   Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-X1-Neo-HA-SEC62

    Plasmid

    #139865

    Purpose

    Lentiviral expression of HA-tagged human SEC62

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    SEC62 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 Flag TSC2 (S939A/T1462A)

    Plasmid

    #14131

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A/T1462A (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A/T1462A

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Promoter

    Ptet

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-beta-3

    Plasmid

    #27289

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin beta 3 (ITGB3 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1-alpha-IIb

    Plasmid

    #27288

    Depositor

    Timothy Springer

    Article

    Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

    Insert

    integrin alpha IIb (ITGA2B Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TetOn-REEP5-mCherry-eGFP

    Plasmid

    #139870

    Purpose

    Dox-inducible lentiviral expression of human REEP5

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    REEP5 (REEP5 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PH-PLCD1(R40L)-GFP

    Plasmid

    #51408

    Purpose

    Non-PI(4,5)P2 binding control for PH-PLCD1-GFP

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 40 mutated to leucine

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pGal-BE3

    Plasmid

    #172409

    Purpose

    Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

    Depositor

    Leonid Kruglyak

    Article

    Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

    Insert

    BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GALL

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a/Cas9-Cys

    Plasmid

    #53261

    Purpose

    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

    Depositor

    Hyongbum Kim

    Article

    Ramakrishna et al Genome Res. 2014 Apr 2.

    Insert

    Cas9-Cys
    

    Tags

    6X His tag, HA epitope, and NLS

    Expression

    Bacterial

    Mutation

    Additional Cysteine is added to the C-terminal re…

    Promoter

    T7 Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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