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pAP2050-2

Plasmid

#105243

Purpose

mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAE-MIBP1

Plasmid

#51873

Purpose

Expresses full length rat MIBP1 (cloned in pCAGGS)

Depositor

Tomoko Tahira, Kenshi Hayashi

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pUASt-FMW-attB-AthHen1

Plasmid

#104958

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

Hsp70 promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-S437A

Plasmid

#208803

Purpose

Xenopus laevis His6-Myc-DONSON (S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

S437A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1 (M50P)

Plasmid

#178651

Purpose

Mammalian expression of myc-tagged MR1 (M50P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

M50P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (H83P)

Plasmid

#178652

Purpose

Mammalian expression of myc-tagged MR1 (H83P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

H83P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (Q153P)

Plasmid

#178653

Purpose

Mammalian expression of myc-tagged MR1 (Q153P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Q153P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMIGR AMPK WT

Plasmid

#27564

Depositor

Morris Birnbaum

Insert

AMP Acticated Protein Kinase (Prkaa2 Rat)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pLEX SC4MOL

Plasmid

#27983

Depositor

Igor Astsaturov

Insert

SC4MOL (MSMO1 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pAGNT-Sema3A

Plasmid

#16428

Depositor

Jonathan Raper

Insert

Sema3A (SEMA3A Chicken)

Use

Tags

His and Myc

Expression

Mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pCAGGs-NLS-TIR1_P2A_NES-TIR1

Plasmid

#117699

Purpose

Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

TRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

HA tag, Myc-NES, and NLS

Expression

Mammalian

Mutation

codon optimized for murine expression

Promoter

None, 2A peptide from porcine teschovirus-1 polyp…

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSBinit

Plasmid

#110100

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Use

Tags

myc, His

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Article

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL1-pCMV7.1

Plasmid

#155019

Purpose

N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 1 (CUL1 Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

Plasmid

#232122

Purpose

A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

Tcf4ΔN (Tcf4 Mouse)

Use

Gateway cloning

Tags

myc

Expression

Mutation

lacks aa 1-56

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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hGATA-1 WT

Plasmid

#118352

Purpose

This plasmid expresses human GATA-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4B-pCMV7.1

Plasmid

#155023

Purpose

N-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4B (CUL4B Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL3-pCMV7.1

Plasmid

#155021

Purpose

N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 3 (CUL3 Human)

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

pCMV7.1

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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