We narrowed to 26,310 results for: STI;

1. pAV0746

   Plasmid

   #133480

   Purpose

   Expression in fission yeast from promoter prga3 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSW377 - pTMO5

   Plasmid

   #115988

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pTMO5
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. DeltaNIRD C95S KVS-1

   Plasmid

   #113068

   Purpose

   Expresses worm truncated KVS1.2 C113S in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

   Insert

   C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C113S+first 40 AA deletion

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CTL2 (M2225R)

   Plasmid

   #96952

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 (M222R) of PIEZO1
   

   Use

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

   Plasmid

   #91945

   Purpose

   Bacterial expression of maize SAE1 and truncated SAE2

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   HA-ZmSAE1
   ZmSAE2-FLAG
   

   Use

   Tags

   FLAG and HA

   Expression

   Bacterial

   Mutation

   Truncation of ZmSAE2 to mimic a splice variant

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Tag/S46X with 5'UTR

   Plasmid

   #87729

   Purpose

   Expresses S46X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S46X

   Promoter

   CMV

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Tag/S44L with 5'UTR

   Plasmid

   #87727

   Purpose

   Expresses S44L spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S44L

   Promoter

   pCMV

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Tag/E112K with 5'UTR

   Plasmid

   #87723

   Purpose

   Expresses E112K spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E112K

   Promoter

   CMV

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag/L195V with 5'UTR

   Plasmid

   #87725

   Purpose

   Expresses L195V spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L195V

   Promoter

   CMV

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Tag 3A/myc-M1E112K

    Plasmid

    #87715

    Purpose

    Expresses myc-M1E112K spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M1 isoform; E112K

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. const #7-RFP

    Plasmid

    #81081

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23109

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pconst #6-RFP

    Plasmid

    #81080

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23117

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBGSA FPR1 N297A

    Plasmid

    #66723

    Purpose

    Constitutive expression of FPR1 N297A in mammalian cells.

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

    Insert

    FPR1 (haplotype 3) (FPR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N297A

    Promoter

    SR-alpha

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

    Plasmid

    #136262

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2 8xSTAR-sTomato-NLS. PGK-puro

    Plasmid

    #136261

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-mEGFP(A206K)-paCaMKII(T286A)

    Plasmid

    #165439

    Purpose

    Expresses photoactivatable CaMKII(T286A: Autophosphorylation deficient) in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)
    

    Use

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    T286A: Autophosphorylation deficient mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV Blast DEST (706-1)

    Plasmid

    #17451

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-CMV-PRB

    Plasmid

    #206165

    Purpose

    pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    Progesteron receptor B (PGR Human)
    

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC_mNG2(11)_BSDminus_F2

    Plasmid

    #184164

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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