We narrowed to 15,050 results for: pET

1. pETM-60-HsAGO2-448-859-optim_K

   Plasmid

   #146729

   Purpose

   Bacterial Expression of HsAGO2_448-859-optim

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO2_448-859-optim (AGO2 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM-60-NusA-DmEDC3_331-400-ADA_H

   Plasmid

   #146447

   Purpose

   Bacterial Expression of DmEDC3_331-400-ADA

   Depositor

   Elisa Izaurralde

   Insert

   DmEDC3_331-400-ADA (Edc3 Fly)
   

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a(+)-His(6)-TEV-AtPCO5(wt)

   Plasmid

   #191803

   Purpose

   Full length wt AtPCO5 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO5
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM-41P-HsXRN1_1174-1649-Strep_AH

   Plasmid

   #148901

   Purpose

   Bacterial Expression of HsXRN1_1174-1649

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

   Insert

   HsXRN1_1174-1649 (XRN1 Human)
   

   Expression

   Bacterial

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PLS-KP3-C93A-C96A_rpoA-U

   Plasmid

   #190962

   Purpose

   Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP3
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:C93A/C96A

   Promoter

   T7

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-PLS-KP6-C93A-C96A_rpoA-C

   Plasmid

   #190970

   Purpose

   Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:C93A/C96A

   Promoter

   T7

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-PLS-KP2-C93A-C96A_rpoA-U

   Plasmid

   #190958

   Purpose

   Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:C93A/C96A

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-PLS-KP6-C93A-C96A_rpoA-U

   Plasmid

   #190971

   Purpose

   Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:C93A/C96A

   Promoter

   T7

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mouse KANK3 Ankyrin Repeats pET151

   Plasmid

   #164056

   Purpose

   Expresses mouse KANK3 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 3 (Kank3 Mouse)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mouse KANK4 Ankyrin Repeats pET151

    Plasmid

    #164057

    Purpose

    Expresses mouse KANK4 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Duet1-6xHis-TEV-NDP52

    Plasmid

    #190194

    Purpose

    Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

    Plasmid

    #186274

    Purpose

    Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas6-RT-Cas1
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Duet1-6xHis-TEV-OPTN

    Plasmid

    #190192

    Purpose

    Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-His6-Sumo-CylK-Strep

    Plasmid

    #185962

    Purpose

    Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

    Depositor

    Emily Balskus

    Article

    Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

    Insert

    CylK
    

    Tags

    His6-Sumo

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet1-LanCL2-H264A-C-Ter-His

    Plasmid

    #154187

    Purpose

    To express LanCL2-H264A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His264->Ala264

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET Duet1 His6 SUMO D14

    Plasmid

    #177854

    Purpose

    Bacterial Expression of D14

    Depositor

    Karsten Melcher

    Article

    Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

    Insert

    D14
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET24a His6 GST PYL2(14-188)

    Plasmid

    #177842

    Purpose

    Bacterial Expression of PYL2

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 2 (PYL2 Mustard Weed)
    

    Tags

    6xHis-GST

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-Fgβ-anticalin-His-ybbr

    Plasmid

    #168040

    Purpose

    Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Tags

    6x Histag, Fgβ peptide, and ybbr tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b p97-Strep-His E314Amb

    Plasmid

    #169021

    Purpose

    Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Tags

    His and SBP

    Expression

    Bacterial

    Mutation

    E314TAG

    Promoter

    T7

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b-His-TEV-SNAP-tag_dead_mt

    Plasmid

    #167270

    Purpose

    Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    C145A

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. CPH3567 (pET151_Spt6 1247-1451 R1282H)

    Plasmid

    #91816

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed arginine 1282 to histidine; deleted resid…

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. CPH3495 (pET151_Spt6 1247-1451 K1435A)

    Plasmid

    #91817

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1435 to alanine; deleted residues …

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-11a (GB1 WT - A34F/V39L)

    Plasmid

    #136610

    Purpose

    Expresses GB1 WT double mutant (A34F/V39L)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    GB1 WT double mutant (A34F/V39L)
    

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: A34F - V39L

    Promoter

    T7 promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. AAV-Synapsin-Ypet-3XNLS-WPRE

    Plasmid

    #159950

    Purpose

    nucleus marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-3XNLS
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. AAV-Synapsin-Ypet-optoJNKi-WPRE

    Plasmid

    #159967

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-optoJNKi
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET-45b-HOXA13(+7A)-IDR-mCherry

    Plasmid

    #145285

    Purpose

    Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(+7A)-IDR (HOXA13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET-45b-HOXA13(WT)-IDR-mCherry

    Plasmid

    #145284

    Purpose

    Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(WT)-IDR (HOXA13 Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET15-His-∆NGlpG(C104A/M247C)

    Plasmid

    #155133

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/M247C, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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