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pcDNA6.MCV.LT339.V5.4

Plasmid

#28193

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCPyV Truncated Tumor Large T antigen (399 tumor strain)

Tags

V5

Expression

Bacterial and Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

Plasmid

#64112

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-PAmCherry1-MCS

Plasmid

#60608

Purpose

Cloning PAmCherry1

Depositor

Steven Chu, Xiaolin Nan

Article

Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;11

Insert

PAmCherry1

Use

Cloning

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIIG

Plasmid

#186828

Purpose

Expresses FLAG-CaMKIIG in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2g (Camk2g Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_NT135

Plasmid

#183121

Purpose

pLentiGuide modified to express mCherry and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Puro.let-7gsm.Cre

Plasmid

#17410

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

Let-7g mutant

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

mutated version of let-7g

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-tLuc-mCherry

Plasmid

#107553

Purpose

tLuc-mCherry in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Type

Empty backbone

Use

AAV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-Nipah V

Plasmid

#174228

Purpose

Expresses Nipah virus V

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

Nipah V

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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pKL004

Plasmid

#98920

Purpose

Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

GCaMP6f tethered to mRuby

Tags

His and mRuby3

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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p416 25Q GAL

Plasmid

#1185

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh20.1 GFP

Plasmid

#209106

Purpose

GFP expressing scramble of shRNA targeting Cdh20

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh20.1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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p4028 GST-E6AP

Plasmid

#8655

Depositor

Peter Howley

Article

Oda et al Proc Natl Acad Sci U S A 1999 Aug 17;96(

Insert

E6-AP (UBE3A Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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35S-DsREDmonomer-NosT

Plasmid

#79183

Purpose

Transient expression vector for DsRED monomer

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Type

Empty backbone

Use

Reporter plasmid

Tags

DsREDmonomer

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Wild-type CoV-2 ORF8

Plasmid

#159933

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHW533 (Peft-3::cGAL-N::let-858 3'UTR)

Plasmid

#107132

Purpose

cGAL-N driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans eft-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHW531 (Peft-3::cGAL-C::let-858 3'UTR)

Plasmid

#107136

Purpose

cGAL-C driver under the control of C. elegans eft-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans eft-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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