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pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

Plasmid

#231128

Purpose

Expresses xylanase via a cSAT2.0 scheme

Depositor

Xiaofeng Yang

Article

Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

Insert

xylanase

Tags

cSAT2.0

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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GluA2 (px330:2-guideCas9)

Plasmid

#169420

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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GluN1 (px330:2-guideCas9)

Plasmid

#169426

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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rGluA1 (px330:2-guideCas9)

Plasmid

#169437

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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p4458 FLAG-hPLIC-1

Plasmid

#8663

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-1 (UBQLN1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
Cas9m4-VP64

Plasmid

#47319

Purpose

Cas9m4 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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UAS-3xHalo7::CAAX

Plasmid

#87646

Purpose

For creating drosophila transgenics expressing UAS-3xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

3xHalo7::CAAX

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC-FLAG-dN6-His

Plasmid

#22143

Depositor

Robert Sauer

Article

Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Insert

Flag-clpXdeltaNLinkedHexamer-His6

Tags

Flag, His6, and L20 Linkers

Expression

Bacterial

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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GluA1 (px330:2-guideCas9)

Plasmid

#169417

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Positron

Plasmid

#129253

Purpose

Express Positron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet

Plasmid

#125134

Purpose

Natively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub

Plasmid

#232577

Purpose

Express Ubiquitin tagged with an Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP(-3)-Ub

Plasmid

#232583

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAMBIA_ASX

Plasmid

#98888

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Article

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

CaMV35S (enhanced)

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLSSmKate1-N1

Plasmid

#31866

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate1

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Flag-SmBiT-PP1b

Plasmid

#224895

Purpose

Mammalian expression of Flag-SmBiT-PP1b

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

Flag-SmBiT-PP1b

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA23-GG

Plasmid

#208013

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Article

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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