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Flag-Gsdmd-NT 4A

Plasmid

#80952

Purpose

Expresses Gsdmd-NT 4A mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT 4A (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCytERM_mScarlet_N1

Plasmid

#85066

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV LDTS-eLIR

Plasmid

#189000

Purpose

lipophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-eLIR

Use

Retroviral

Tags

HA

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::CreERT2-bGHpA

Plasmid

#62444

Purpose

MXS_chaining vector with PGK::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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CAG sHRPa-NRX

Plasmid

#73143

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Tags

2x HA tag and Flag

Expression

Mammalian

Promoter

CAG

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs N-terminal sgRNA

Plasmid

#207093

Purpose

pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGCGGGACTCGGCGGCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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MCP-GFP1-10 P2A PCP-GFP11

Plasmid

#162038

Purpose

Background-free mRNA labeling bipartite superfolder GFP system

Depositor

Hye Yoon Park

Article

Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

Insert

MCP-GFP1-10 P2A PCP-GFP11

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP1-10-miniCMV-GFP11×11-GFP-tDeg

Plasmid

#185404

Purpose

Expresses GFP1-10 and GFP11×11-GFP-tDeg

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

GFP1-10

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCA11-pMa-sgTS3-17xMS2V5-sgTS3

Plasmid

#199442

Purpose

CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

HITI donor template

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP713-N1

Plasmid

#136559

Purpose

A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pmEGFP-DRD2

Plasmid

#190752

Purpose

Expression of DRD2 with mEGFP tag on N-terminus.

Depositor

Dave Piston

Article

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

DRD2 (DRD2 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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ArcLight-A242

Plasmid

#36857

Depositor

Vincent Pieribone

Article

Jin et al Neuron. 2012 Sep 6;75(5):779-85.

Insert

fluorescent protein voltage sensor

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

semian CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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PCDNA3-GFP1-9 T2A mCherry

Plasmid

#124430

Purpose

Expresses GFP1-9 and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

GFP1-9 T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205551

Purpose

Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

Plasmid

#158793

Purpose

Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Neuraminidase A/NL/109/03

Tags

TwinStrep_sfGFP_tetrabrachion

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Expression

Worm

Promoter

rab-3

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-S(4+)-EGFP

Plasmid

#21317

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-S(4+) promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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