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HLTV-hTRIM21

Plasmid

#104973

Purpose

Purification of His-Lipoyl-hTRIM21 protein from bacteria

Depositor

Leo James

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Tags

His-Lipoyl

Expression

Bacterial

Promoter

T7

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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EGFP-CAAX

Plasmid

#86056

Purpose

A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Homo sapiens Harvey rat sarcoma (HRAS Human)

Tags

GFP

Expression

Mammalian

Mutation

contains the region from 825-857 of HRAS as a far…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pDECKO_mCherry

Plasmid

#78534

Purpose

Backbone to clone single or paired sgRNAs

Depositor

Roderic Guigo, Rory Johnson

Publication

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA)

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Publication

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pAAV-hSyn-dF-HA-KORD-IRES-mCitrine

Plasmid

#65417

Purpose

AAV packaging vector for Cre-dependent, hSyn driven KOR DREADD expression.

Depositor

Bryan Roth

Publication

Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

Has service

AAV8 and AAV Retrograde

Insert

hSyn-dF-HA-KORD-IRES-mCitrine (OPRK1 Human)

Use

AAV

Tags

HA and mCitrine

Promoter

hSyn

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCAG-VSVP

Plasmid

#64088

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

Depositor

Ian Wickersham

Insert

VSV phosphoprotein

Expression

Mammalian

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Publication

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pVITRO1-dV-IgG1/κ

Plasmid

#52213

Purpose

Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Publication

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC1-REX-YFP

Plasmid

#48247

Purpose

Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Publication

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

REX-YFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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PU6::unc-119_sgRNA

Plasmid

#46169

Purpose

unc-119 targeting sgRNA

Depositor

John Calarco

Publication

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

unc-119 targeting sgRNA (unc-119 Synthetic)

Use

CRISPR

Promoter

C. elegans U6 snRNA pol III promoter

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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pSpliceExpress

Plasmid

#32485

Depositor

Stefan Stamm

Publication

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pLKO-puro FLAG SREBP2

Plasmid

#32018

Depositor

David Sabatini

Publication

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

SREBP2 (Srebf2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway Cloning

Mutation

M260I

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Lenti-LucOS

Plasmid

#22777

Depositor

Tyler Jacks

Insert

OVA-SIY

Use

Cre/Lox and Lentiviral

Tags

luciferase

Expression

Mammalian

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDisplay-AP-CFP-TM

Plasmid

#20861

Depositor

Alice Ting

Publication

Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

AP-CFP-TM

Tags

CFP, HA, and myc

Expression

Mammalian

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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