We narrowed to 23,108 results for: CRISPR

1. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SMVP-Cas9N

   Plasmid

   #80930

   Purpose

   Expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT01

   Plasmid

   #223373

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpdCas9 VPR

   Plasmid

   #188509

   Purpose

   Expresses FLAG tagged SpdCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2CT-His-MBP-Lbu_C2c2_WT

   Plasmid

   #83482

   Purpose

   Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFnCpf1GG

   Plasmid

   #131010

   Purpose

   Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of FnCas12a
   GFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM068

   Plasmid

   #216811

   Purpose

   Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Type

   Empty backbone

   Use

   CRISPR; Fungal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_MCP-LSD1_Hygro

    Plasmid

    #138457

    Purpose

    lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    LSD1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFYF1327 EGFP Site#2

    Plasmid

    #47512

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2CT-His-MBP-Lwa_Cas13a_WT

    Plasmid

    #91865

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LwaCas13a
    

    Use

    CRISPR

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEn-Chimera

    Plasmid

    #61432

    Purpose

    Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AsCas12a

    Plasmid

    #188492

    Purpose

    Expresses FLAG tagged AsCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-MS2-Tet1-CD

    Plasmid

    #83341

    Purpose

    To introduce MS2 coat protein fused Tet1-CD

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    MS2-Tet1-CD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDe-CAS9-D10A

    Plasmid

    #61477

    Purpose

    based on pDe-CAS9, encodes Cas9 D10A nickase variant

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    catalytic Asp10 changed to Ala

    Promoter

    PcUbi4-2

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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