We narrowed to 3,684 results for: Stom

1. pXPR_051_dCas9-KRAB-HA

   Plasmid

   #228936

   Purpose

   All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUS2-SH231

   Plasmid

   #115150

   Purpose

   Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Dual SH231 gRNAs
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mEGFP-CaMKIIa (T286A)

   Plasmid

   #127390

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   pCAG

   Available since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. EHD2-mEGFP

   Plasmid

   #45932

   Purpose

   caveolar protein, confines caveolae to the plasma membrane

   Depositor

   Ari Helenius

   Article

   Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:

   Insert

   Eps-15 homology domain-containing protein2 (EHD2 Human)
   

   Use

   Tags

   monomeric EGFP_FrameN1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINTO-NFH::hSNRNP70

   Plasmid

   #65926

   Purpose

   Expression of human SNRNP70 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70 Human)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Two silent mutations

   Promoter

   CMV/TO and CMV/TetO2 (T-REx)

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-REX-GECO1

   Plasmid

   #61246

   Purpose

   Expresses REX-GECO1 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

   Insert

   REX-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: P60R, V61W, R6…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-eGFP_mito

   Plasmid

   #221414

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   eGFP_mito
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Circular 200,100 with interspersed loops (FANCC)

   Plasmid

   #180192

   Purpose

   AAV vector carrying a guide RNA targeting the human FANCC mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Circular 200,100 with interspersed loops (CTNNB1)

   Plasmid

   #180193

   Purpose

   AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-eGFP_KDEL

    Plasmid

    #221411

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP_KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

    Plasmid

    #165435

    Purpose

    Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. Lenti-BaeI-Pgk-Cre

    Plasmid

    #173663

    Purpose

    Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    BaeI site
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAX198

    Plasmid

    #173042

    Purpose

    Custom vector for sgRNA library construction

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDG335

    Plasmid

    #100899

    Purpose

    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV HA hRB delta CDK + S612

    Plasmid

    #58914

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S612 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV HA hRB delta CDK + S608

    Plasmid

    #58913

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S608 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. H14_MBP_SUMO-Cys-linker-BAF

    Plasmid

    #101777

    Purpose

    Expresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Tags

    His14-MBP-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42

    Plasmid

    #165434

    Purpose

    Expresses ShadowY-CBD and Clover(T153M/F223R)-Cdc42 in mammalian cells to monitor Cdc42 activity with FLIM imaging.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    ShadowY-CBD-P2A-Clover(T153M/F223R)-Cdc42
    

    Use

    Tags

    ShadowY, Clover(T153M/F223R)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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