We narrowed to 4,271 results for: pme
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Plasmid#104721PurposeUAS-driven expression of activated MEKDepositorInsertactivated map2k2b (map2k2b Zebrafish)
UseZebrafish transposonTags2A-tdTomato and myc tagMutationserines 221 and 225 to aspartic acidPromoter10xUASAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-UnaG-P2A-FusionRed
Plasmid#191109PurposeMammalian cell line expression plasmids for production of UnaG and FusionRed proteinsDepositorInsertUnaG
UseAAVTagsFusionRedExpressionMammalianMutationSee depositor comments belowPromoterchicken β-actin promoterAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR
Plasmid#129774PurposeMarker of microtubule dynamics in C. elegans hypodermisDepositorInsertsUseSynthetic Biology; GatewayTagsSynthetic Intron and mGFPExpressionWormPromoterPcol-19Available SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
612 p3E-2A-mCherryCAAXpA
Plasmid#195971PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCherry-CAAX (membrane)
UseOtherAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
ExpressionBacterialMutationS226P/R228SAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP
Plasmid#234881PurposeL1HS-silencing plasmid (CRISPRi gRNA2)DepositorInsertLacZ gRNA
UseCRISPR and LentiviralTagsGFPExpressionMammalianAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-EGFP-TM-IRES-Pac
Plasmid#183604PurposeMammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
TagsHuman PDGFRB transmembrane domain and Mouse IgGK …ExpressionMammalianPromoterMouse Pgk1Available SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mKdm4d-polyA
Plasmid#61553PurposeFor mRNA synthesis or expression in mammalian cellsDepositorAvailable SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
STOP-eGFP-ROSA26TV
Plasmid#11739DepositorInsertSTOP-eGFP-ROSA26TV
UseCre/Lox; /stopAvailable SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
Puro-iNotch1IC
Plasmid#75338Purposedoxycycline inducible Notch1 intracellular domain gene over-expressionDepositorAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
766 p3E-2A-nlsmCherrypA
Plasmid#195972PurposeGateway p3E 2A fluorophoreDepositorInsert2A-nlsmCherry (nuclear)
UseOtherAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUASp-GBP-Tbro
Plasmid#179995PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.DepositorInsertGBP nanobody
TagsTrboExpressionInsectPromoterUAS-Gal4Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPP6
Plasmid#209968PurposeContains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertionDepositorInsertPromoter Dropout (sfGFP)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
TagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCR4-beta-globin::lacZ-H11
Plasmid#227000PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingExpressionMammalianPromoterbeta-globinAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272
Plasmid#154834PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanerceptDepositorInsertsETN
ETN
UseCre/LoxExpressionMammalianPromoter100RPU.2Available SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGMCtKq28-TSC10M1-sspB
Plasmid#49526PurposeKanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety siteDepositorInsertTSC10M1-sspB
ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only