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20,362 results

Showing: 2701 - 2720 of 20362 results

  1. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Publication
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104992-plasmid-map-sequence-id-213507

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  2. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Publication
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112203-plasmid-map-sequence-id-473446

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  3. pX330A-nBRD4/PITCh

    Plasmid
    #91794
    Purpose
    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chicken β-actin promoter
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91794-plasmid-map-sequence-id-429087

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  8. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    72266-plasmid-map-sequence-id-447561

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  9. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Publication
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63891-plasmid-map-sequence-id-450336

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  10. pCMV CEPIA4mt

    Plasmid
    #58220
    Purpose
    Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)
    Depositor
    Masamitsu Iino
    Publication
    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CEPIA4mt
    Tags
    myc
    Expression
    Mammalian
    Mutation
    GCaMP2 N263Y E282V E334D M339L F395W D436E
    Promoter
    CMV
    Available since
    July 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58220-plasmid-map-sequence-id-274391

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Showing: 2701 - 2720 of 20362 results