19,634 results

1. pORANGE Cloning template vector

   Plasmid

   #131471

   Purpose

   Cloning template form ORANGE method based knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Tags

   HA

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK7-HA-S6K1-E389-deltaCT

   Plasmid

   #8993

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T389E, stop codon at amino acid 402

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. gag-COM-pol

   Plasmid

   #211373

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-COM-pol
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AYBEv3

   Plasmid

   #193967

   Purpose

   An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(v3)
   

   Expression

   Mammalian

   Mutation

   G163R, N169S, S198A, K202A, G203A, S206A, K210A

   Promoter

   hU6, EF1a, CBh

   Available Since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. InsRA-eGFP

   Plasmid

   #79795

   Purpose

   Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   InsRA (INSR Human)
   

   Tags

   eGFP extracellular

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD-DsRed

   Plasmid

   #51434

   Purpose

   Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

   Depositor

   Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   LoxP-3xP3-DsRed-LoxP
   

   Promoter

   3xP3

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-hnRNPU-V5

   Plasmid

   #35974

   Depositor

   Susana Valente

   Article

   Mediouni et al Curr HIV Res. 2015;13(1):64-79.

   Insert

   heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. V5-GFP-TurboID-KDEL_pLX208

   Plasmid

   #202020

   Purpose

   expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-TurboID-KDEL
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pS2513·PHP

   Plasmid

   #122590

   Purpose

   Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

   Depositor

   Pablo Ivan Nikel

   Article

   Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

   Insert

   phIorine2
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pem7

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Murine E-cadherin mCherry

    Plasmid

    #71366

    Purpose

    mammalian expression of murine E-caderin with mCherry fusion

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    E-cadherin (Cdh1 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6-SpyCatcher-mi3

    Plasmid

    #113043

    Purpose

    Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    His6-SpyCatcher-mi3
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVasa-Cre

    Plasmid

    #15885

    Depositor

    Diego Castrillon

    Article

    Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

    Insert

    mouse vasa promoter (Ddx4 Mouse)
    

    Tags

    Cre

    Expression

    Mammalian

    Available Since

    Aug. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Plasmid

    #179115

    Purpose

    Cargo EGFP with AttP Bxb1 site (Parent minicircle)

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    Cargo EGFP with AttP Bxb1 site
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-LC3

    Plasmid

    #11546

    Depositor

    Karla Kirkegaard

    Article

    Jackson et al PLoS Biol. 2005 May . 3(5):e156.

    Insert

    LC3B (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-a1AR
    

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-multi-Guide

    Plasmid

    #85401

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xMma PylT_FLAG-Mma PylRS

    Plasmid

    #140009

    Purpose

    Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-NbALFA

    Plasmid

    #159987

    Purpose

    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mCherry-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mCherry and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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