We narrowed to 9,355 results for: bli

1. pHA AKT2 T309A/S474A

   Plasmid

   #60128

   Purpose

   expresses a mutant AKT2

   Depositor

   Morris Birnbaum

   Insert

   protein kinase B (AKT2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   Serine 474 and Threonine 309 changed to alanines

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. VRK1

   Plasmid

   #39067

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   VRK1 (VRK1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Surface mutant for crystallisation K34A, K35A, E3…

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. EPB41L3A-c000

   Plasmid

   #175458

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-D1087.

   Depositor

   Opher Gileadi

   Insert

   EPB41L3 (EPB41L3 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-TMPRSS11D-blast

   Plasmid

   #158476

   Purpose

   Constitutive lentiviral expression of human TMPRSS11D.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   TMPRSS11D (TMPRSS11D Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2

   Plasmid

   #51092

   Purpose

   Forebrain principal neuron expression of oChIEF kinetic variant E163A/T199C with physically uncoupled mKate2 red fluorophore

   Depositor

   Jonathan Ting

   Insert

   oChIEF(E163A/T199C)
   

   Use

   AAV

   Tags

   P2A-mKate2

   Expression

   Mammalian

   Mutation

   E163A/T199C

   Promoter

   CaMKIIa

   Available Since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti puro HA-LMNB1

   Plasmid

   #227591

   Purpose

   Lentiviral plasmid expressing human LMNB1 protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   LMNB1 (LMNB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

   Plasmid

   #68347

   Purpose

   AAV plasmid expressing the FlpO recombinase. with empty gRNA

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   FlPO
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-CTSS-puro

   Plasmid

   #158469

   Purpose

   Constitutive lentiviral expression of human CTSS.

   Depositor

   Sho Iketani, Alejandro Chavez

   Insert

   CTSS (CTSS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. KLHL11

   Plasmid

   #39019

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KLHL11 (KLHL11 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. AICSDP-77:TERF2-mEGFP

    Plasmid

    #168798

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human TERF2.

    Depositor

    Allen Institute for Cell Science

    Insert

    TERF2 (TERF2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    April 14, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3.1-MAP3K5 (R256C)

    Plasmid

    #47105

    Purpose

    Expresses mutant (R256C) human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 256 to cysteine

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. MSCV-FLAG-mMdm2-IRES-GFP

    Plasmid

    #66955

    Purpose

    Retroviral vector to express a FLAG-tagged mouse Mdm2

    Depositor

    Martine Roussel

    Insert

    Mdm2 (Mdm2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid

    #71659

    Purpose

    Mammalian expression of GEMIN6

    Depositor

    Gideon Dreyfuss

    Insert

    GEMIN6 (GEMIN6 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pNY-351

    Plasmid

    #231048

    Purpose

    Used for staining GFP

    Depositor

    Jeremy Nathans

    Insert

    anti GFP DARPin fused to goat Fc
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-TMPRSS2

    Plasmid

    #158441

    Purpose

    Gateway Cloning compatible entry vector for the human TMPRSS2 gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4IJ8

    Plasmid

    #51327

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJ8 (KMT5A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. Mouse-Dysferlin-Transcript-Variant1

    Plasmid

    #60214

    Purpose

    Mouse dysferlin transcript variant-1 (accession #NM_021469) cDNA in pDONR221 plasmid which can be used as an entry vector for Invitrogen’s gateway system.

    Depositor

    Jain Foundation

    Insert

    Dysferlin (Dysf Mouse)
    

    Use

    Gateway entry clone

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEU-Gm23

    Plasmid

    #53738

    Purpose

    Used for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences system

    Depositor

    Gabor Igloi

    Insert

    arginyl-tRNA synthetase
    

    Use

    Synthetic Biology

    Tags

    His tag

    Promoter

    SP6

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLK2

    Plasmid

    #39063

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CLK2 (CLK2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. GSG2

    Plasmid

    #38915

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GSG2 (HASPIN Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. CAPZA1-TwinStrepII

    Plasmid

    #83193

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    CAPZA1 (Capza1 Mouse)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CSNK1G1

    Plasmid

    #38934

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CSNK1G1 (CSNK1G1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

23. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

    Plasmid

    #191527

    Purpose

    Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG) + 3xFLAG_TwinStrep

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCLIPf-NK1R Control Plasmid

    Plasmid

    #101125

    Purpose

    Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Neurokinin-1 Receptor, Truncated (TACR1 Human)
    

    Tags

    10xHis, CLIP-tag (CLIPf), and FLAG Epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pALPS-ZsGreen-miR-146a

    Plasmid

    #89174

    Purpose

    encodes human/murine miR-146a and ZsGreen1

    Depositor

    Hilary Sheppard

    Insert

    miR-146a (MIR146A, Mir146 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    SFFVp

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. mT2A-sfGFP-linker-iCre-ERT2-FNF

    Plasmid

    #182341

    Purpose

    Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-sfGFP-linker-iCre-ERT2-FNF
    

    Use

    Mouse Targeting; Flp / frt

    Expression

    Mammalian

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. mCherry-Galectin-C-18

    Plasmid

    #62745

    Purpose

    Localization: Membrane Receptor, C1 Cloning Vector, Excitation:587, Emission:610

    Depositor

    Michael Davidson

    Insert

    Galectin (LGALS1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid

    #110568

    Purpose

    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag

    Depositor

    Alexander Nectow, Jeffrey Friedman

    Insert

    3xFLAG/HA-tagged anti-GFP VHH
    

    Use

    AAV, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGFP CrkII

    Plasmid

    #50730

    Purpose

    mammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    CrkII (CRK Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pMT-Flag-RELISH

    Plasmid

    #63750

    Purpose

    Expresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    Relish (Rel Fly)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    pMT

    Available Since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIC144

    Plasmid

    #63067

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-K, IMAGE clone 3511065 (CENPK Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHH0103 BAG3 WW domain

    Plasmid

    #104266

    Purpose

    Bacterial expression of WW domain from BAG3

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    BAG3 WW domain (BAG3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FDX1

    Plasmid

    #39055

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FDX1 (FDX1 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pLV hMyoD-IRES-dsRed

    Plasmid

    #66631

    Purpose

    Co-expresses human MyoD and dsRed

    Depositor

    Charles Gersbach

    Insert

    human MyoD IRES dsRed (MYOD1 Human)
    

    Use

    Lentiviral

    Promoter

    hUbc

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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35. pCXLE_hePOU_shp53

    Plasmid

    #206397

    Purpose

    Integration-free (episomal) expression of human ePOU and shRNA against p53.

    Depositor

    Ralf Jauch

    Inserts

    ePOU
    shRNA against p53 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLEX307-TMPRSS11D-puro

    Plasmid

    #158475

    Purpose

    Constitutive lentiviral expression of human TMPRSS11D.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS11D (TMPRSS11D Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEF-GalNAcT2-moxGFP2

    Plasmid

    #218966

    Purpose

    Expresses full length GalNAcT2-moxGFP2 mammalian cells

    Depositor

    Benjamin Glick

    Insert

    GalNAcT2-moxGFP2 (GALNT2 Human)
    

    Tags

    moxGFP2

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCHA_OROV_NSm

    Plasmid

    #208940

    Purpose

    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSm
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 949 bp from 5' end (Gn), deleted 29…

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNHA_OROV_NSm

    Plasmid

    #208935

    Purpose

    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSm
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 949 bp from 5' end (Gn), deleted 29…

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mTagBFP2-MCAK-C-7

    Plasmid

    #62785

    Purpose

    Localization: Microtubules/Kinesin, C1 Cloning Vector, Excitation:399, Emission:456

    Depositor

    Michael Davidson

    Insert

    MCAK (KIF2C Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge