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We narrowed to 18,759 results for: nls

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Showing: 5401 - 5440 of 18759 results

  1. pCM67

    Plasmid
    #261137
    Purpose
    deltaPB1 PAR-6::VC155 and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    elt-2p::AID::nls::mcherry::2a::AID::deltaPB1 PAR-6::VC155::tbb-2 3' in pLF3FShC (par-6 Nematode)
    Expression
    Worm
    Mutation
    deltaPB1 PAR-6
    Promoter
    elt-2 minimal promoter
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261137-plasmid-map-sequence-id-520092

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  3. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210734
    Purpose
    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Frodo sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    210734-plasmid-map-sequence-id-464595

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  4. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210735
    Purpose
    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Gollum sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210735-plasmid-map-sequence-id-420080

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  5. ABE9-SpRY

    Plasmid
    #242976
    Purpose
    A-to-G base editor fused to PAM-flexible SpRY-Cas9 nickase.
    Depositor
    Marc Freichel
    Publication
    Ong et al Sci Rep. 2026 Feb 20;16(1):7463. doi: 10
    Insert
    pCMV-TadA-8e (N108Q/L145T)-nSpRYCas9
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    6*His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242976-plasmid-map-sequence-id-486100

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  9. AAVS1-iPE-Plus

    Plasmid
    #214062
    Purpose
    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    TRE-tight
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214062-plasmid-map-sequence-id-429371

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  10. pCMV-PE-Plus

    Plasmid
    #214079
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214079-plasmid-map-sequence-id-429365

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  11. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid
    #212965
    Purpose
    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.
    Depositor
    Roland Rad
    Publication
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx
    Use
    Transposon
    Tags
    P2A-Blasticidin resistance
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212965-plasmid-map-sequence-id-422557

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  12. MCP-M-MLV RT∆RNase H

    Plasmid
    #213749
    Purpose
    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213749-plasmid-map-sequence-id-422192

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  13. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    205247-plasmid-map-sequence-id-408440

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  17. FUGW-APPwt-T2A-mCherry

    Plasmid
    #190803
    Purpose
    Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190803-plasmid-map-sequence-id-374464

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  18. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187956
    Purpose
    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187956-plasmid-map-sequence-id-373523

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  24. LiOn-CMV∞Cre

    Plasmid
    #154018
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Publication
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    Cre-FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154018-plasmid-map-sequence-id-308116

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  28. p-dCas9-HDAC1-Hygro

    Plasmid
    #104409
    Purpose
    transient expression of dCas9-HDAC1 fusion protein
    Depositor
    Stephan Beck
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HDAC1 (HDAC1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104409-plasmid-map-sequence-id-204471

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  30. M3D395A-M14-dCas9

    Plasmid
    #134780
    Purpose
    Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells
    Depositor
    Shu-Bing Qian
    Publication
    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    METTL3/METTL14 methyltransferase domain and inactive dCas9
    Expression
    Mammalian
    Mutation
    D395A
    Promoter
    CMV
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134780-plasmid-map-sequence-id-267083

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  34. pRGEB32-BAR

    Plasmid
    #126072
    Purpose
    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
    Depositor
    Charles Hunter
    Publication
    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126072-plasmid-map-sequence-id-247528

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  36. pEG302-SunTagng-22aa

    Plasmid
    #115345
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome
    Depositor
    Steven Jacobsen
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    115345-plasmid-map-sequence-id-222916

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  40. pX458-ECFP

    Plasmid
    #112220
    Purpose
    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP
    Depositor
    Yinming Liang
    Publication
    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    112220-plasmid-map-sequence-id-215204

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Showing: 5401 - 5440 of 18759 results