We narrowed to 5,868 results for: nls

1. pCB301TOR-CRISPR

   Plasmid

   #164797

   Purpose

   For gene editing in oomycetes

   Depositor

   Miaoying Tian

   Article

   Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

   Inserts

   PsNLS-hSpCas9
   sgRNA expression cassette
   

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   HAM34 and RPL41

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sABE v3.22 Lenti_2

   Plasmid

   #209556

   Purpose

   pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pU6, pCMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBTK527

   Plasmid

   #110605

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. Marathon-RT tetramutant for Split-PE (pEH101)

   Plasmid

   #190109

   Purpose

   Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, D74R, N116K, N197R

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRRL-GFPGO2(NGG)-PGK-Neo

   Plasmid

   #136891

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO2(NGG)
   

   Use

   Lentiviral

   Tags

   2xNLS

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. T5-sgRNA

   Plasmid

   #113655

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter T5 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBTK527a

   Plasmid

   #110606

   Purpose

   BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. attB53-Pac-attB53

   Plasmid

   #183610

   Purpose

   RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   Pac
   

   Use

   Rmce

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-LbCas12a

    Plasmid

    #213743

    Purpose

    For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK1_008

    Plasmid

    #123671

    Purpose

    Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-2xHS4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRRL-mU6-GFPGO-IRES-mScarletI

    Plasmid

    #136895

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    3xNLS on mScarlet

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MTK1_022

    Plasmid

    #123684

    Purpose

    Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-A4 insulator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDA125

    Plasmid

    #74249

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pSTL1

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MTK1_014

    Plasmid

    #123677

    Purpose

    Encodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS'-FRT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRO211

    Plasmid

    #233830

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pTDH3-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-mNeonGreen-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRO261

    Plasmid

    #233831

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pRPL18B-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRPL18B-mNeonGreen-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRO262

    Plasmid

    #233832

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pRNR2-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRNR2-mNeonGreen-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRO263

    Plasmid

    #233833

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pRAD27-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRAD27-mNeonGreen-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRO264

    Plasmid

    #233834

    Purpose

    MoClo-YTK; ConLS/RE cassette; Const. mNG; pREV1-mNeonGreen-tENO2

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pREV1-mNeonGreen-tENO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRO229

    Plasmid

    #233836

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA-v10-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRPL18B-rtTA-v10-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRO230

    Plasmid

    #233837

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA3-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRPL18B-rtTA3-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRO231

    Plasmid

    #233838

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRPL18B-rtTA-v16-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRPL18B-rtTA-v16-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRO246

    Plasmid

    #233839

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRNR2-rtTA-v16-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRNR2-rtTA-v16-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRO247

    Plasmid

    #233840

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pRAD27-rtTA-v16-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRAD27-rtTA-v16-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRO253

    Plasmid

    #233841

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pTDH3-rtTA-v10-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-rtTA-v10-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRO254

    Plasmid

    #233842

    Purpose

    MoClo-YTK; ConLS/R1 cassette; rtTA systems; pTDH3-rtTA3-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-rtTA3-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRO241

    Plasmid

    #233844

    Purpose

    MoClo-YTK; ConLS/R1 cassette; ABA systems; pTDH3-Phlf-NES-PYL1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-Phlf-NES-PYL1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWN057

    Plasmid

    #233852

    Purpose

    MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-AmtR-NES-TIR1-U1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWN075

    Plasmid

    #233856

    Purpose

    MoClo-YTK; ConLS/R1 cassette; Auxin systems; pRPL18B-AmtR-NES-TIR1-U1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRPL18B-AmtR-NES-TIR1-U1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWN076

    Plasmid

    #233857

    Purpose

    MoClo-YTK; ConLS/R1 cassette; Auxin systems; pRNR2-AmtR-NES-TIR1-U1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pRNR2-AmtR-NES-TIR1-U1-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pWN086

    Plasmid

    #233859

    Purpose

    MoClo-YTK; ConLS/R1 cassette; Auxin systems; pTDH3-AmtR-NES-TIR1-U2-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-AmtR-NES-TIR1-U2-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pWN113

    Plasmid

    #233862

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-linker-NS3a-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NES-linker-NS3a-tADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid

    #229973

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - mAGH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mRuby3 only VLP

    Plasmid

    #182429

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSCL.103

    Plasmid

    #184988

    Purpose

    Express Cas9-P2A-Eco1RT

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSCL.72

    Plasmid

    #184984

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker1-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSCL.95

    Plasmid

    #184986

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker2-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSCL.102

    Plasmid

    #184987

    Purpose

    Express -Eco1 RT-P2A-Cas9

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-P2A-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSCL.94

    Plasmid

    #184985

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker2-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge