We narrowed to 20,285 results for: ACE;

1. pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

   Plasmid

   #116884

   Purpose

   FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

   Depositor

   Brian Kuhlman

   Article

   Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

   Insert

   LINXa4(RLR)-Bre1
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

   Promoter

   ADH1

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-GFP11x7-HygR

   Plasmid

   #223676

   Purpose

   To check the split GFP signals in mitochondria (2)

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Nature. 2024 May;629(8012):660-668. doi:

   Insert

   GFP11x7
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSS9

   Plasmid

   #71655

   Purpose

   Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli

   Depositor

   Ryan Gill

   Article

   Bassalo et al ACS Synth Biol. 2016 Apr 22.

   Insert

   gfpuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7A1

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMG343

   Plasmid

   #176183

   Purpose

   preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Insert

   TPK2+ccdB dropout cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nanog5P reporter

   Plasmid

   #16337

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

   Insert

   Nanog promoter (Nanog Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Nanog promoter

   Available Since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestattB/CY (pCM327)

   Plasmid

   #68341

   Purpose

   Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

   Depositor

   Christian Mosimann, Leonard Zon

   Article

   Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

   Type

   Empty backbone

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mEGFP(A206K)-paCaMKII-ShadowG

   Plasmid

   #167475

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GZnP2 in pcDNA3.1+

   Plasmid

   #161739

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

   Insert

   GZnP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBMN HAYFP-LOV24

   Plasmid

   #49570

   Purpose

   regulate protein stability with light

   Depositor

   Thomas Wandless

   Article

   Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

   Insert

   YFP-LOV24
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   delete the last 3 aa from AsLOV2, with I532A muta…

   Promoter

   LTR

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. vYM140_PB-pEFa1-iaaM(P.s)-Hygro

    Plasmid

    #160043

    Purpose

    Expressing iaaM for converting L-Trp to IAM

    Depositor

    Michael Elowitz

    Article

    Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

    Insert

    iaaM (iaaM )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available Since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNOC-ARS-CRISPR-v2

    Plasmid

    #99863

    Purpose

    Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSAR3.5t

    Plasmid

    #210257

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    eBFP2
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

    Plasmid

    #165429

    Purpose

    Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    FHS-paCaMKII
    

    Use

    AAV

    Tags

    FHS(Flag, HisX6, Strep) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII0.4

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Thorsten Mascher, Georg Fritz

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCKan-phoAp-GFPuv

    Plasmid

    #139072

    Purpose

    Low phosphate inducible promoter phoAp for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    phoAp-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Arf Q71L CFP

    Plasmid

    #128149

    Purpose

    Expression of Arf Q71L

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 2002 May 9;417(6885):187-93. doi

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q71L

    Promoter

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTSAR3.1t

    Plasmid

    #210254

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCerulean
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pUAP4

    Plasmid

    #136079

    Purpose

    Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pNAS1B

    Plasmid

    #61968

    Purpose

    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Serine 65 to Glycine (GGT), Serine 72 to Alanine …

    Promoter

    PBAD and PLtetO

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLPO3

    Plasmid

    #209977

    Purpose

    Contains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (pAMBeta1)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pDestattB_ubi:EGFP (pCM272)

    Plasmid

    #68339

    Purpose

    Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    EGFP
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    ubi

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAD-LyseR (in lacZ deficient strain)

    Plasmid

    #99245

    Purpose

    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    phage lambda gene R (R phage lambda)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    beta-lactamase promoter

    Available Since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mNFAT4-462:TALE:VP64:KRɸ

    Plasmid

    #160662

    Purpose

    Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    mNFAT4-462:TALE:VP64:KRɸ
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRISPomyces-1

    Plasmid

    #61736

    Purpose

    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    tracrRNA
    crRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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