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POPDC2 pEGFP-N2 (1087)

Plasmid

#62030

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

POPDC2 (POPDC2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pExpreS2-1-C3-PA

Plasmid

#175446

Purpose

HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

3C-Protein A and BiP Secretion Peptide

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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cmv hTid Short

Plasmid

#13709

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

Plasmid

#109318

Purpose

Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

SaCas9 RuvC gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ub-UBB+1

Plasmid

#23967

Depositor

Nico Dantuma

Article

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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p21 promoter E

Plasmid

#16466

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-HA-GFP

Plasmid

#120903

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ADCK4 gRNA (BRDN0001145019)

Plasmid

#76560

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-Fbxo7(ΔF-box)-IRES-GFP

Plasmid

#171787

Purpose

retrovirus production

Depositor

Heike Laman

Article

Lomonosov et al PLoS One. 2011;6(6):e21165. doi: 10.1371

Insert

Fbxo7 isoform 1 (FBXO7 Human)

Use

Retroviral

Mutation

deletion of aa335-367

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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NKP33 GFP (1032)

Plasmid

#62022

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

C9orf46 (PLGRKT Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
cmv hTid Long

Plasmid

#13707

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
ABE8e(TadA-8e K20A R21A)

Plasmid

#138496

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e K20A R21A)-nSpCas9

Expression

Mammalian

Mutation

TadA-8e K20A R21A

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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p21 promoter C

Plasmid

#16464

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
ADCK3 gRNA (BRDN0001147832)

Plasmid

#77321

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK3 gRNA (BRDN0001146491)

Plasmid

#77323

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-FSF1

Plasmid

#110641

Purpose

Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

FSF1 (FSF1 Budding Yeast)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfTuba_1

Plasmid

#26717

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Tuba RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
p21 promoter G

Plasmid

#16468

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
p21 promoter B

Plasmid

#16463

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
ADRBK2 gRNA (BRDN0001487158)

Plasmid

#77943

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAN1735

Plasmid

#127204

Purpose

Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1

Use

Synthetic Biology

Available Since

June 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Article

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Promoter

PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfTuba_2

Plasmid

#26718

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Tuba RNAi (DNMBP C. familiaris (dog RNAi))

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Tuba_2 RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
p21 promoter D

Plasmid

#16465

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-ATXN1L

Plasmid

#16167

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Similar to Ataxin1 (ATXN1L Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-ODC2

Plasmid

#133257

Purpose

Express FLAG-tagged ODC2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

ODC2 (ODC2 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-NDT2

Plasmid

#133255

Purpose

Express FLAG-tagged NDT2 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NDT2 (YEA6 Budding Yeast)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-ASXL1-HARE-HTH domain

Plasmid

#107164

Purpose

Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

ASXL1 (Asxl1 Mouse)

Tags

Flag

Expression

Bacterial

Mutation

Contains amino acids 2-83

Promoter

T7

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
WFS1 mRFP-N2 (891)

Plasmid

#62018

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

WFS1 (WFS1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
p21 promoter F

Plasmid

#16467

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
CDK4-luciferase active (880)

Plasmid

#86657

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

867 base pairs including up to coding nucleotide …

Promoter

CDK5

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
4E2H.210_LUMABS

Plasmid

#155209

Purpose

Expresses 4E2H.210_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN1779

Plasmid

#127165

Purpose

Vector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)

Depositor

Hana El-Samad

Article

Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1

Use

Synthetic Biology

Available Since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PRPF4B gRNA (BRDN0001146164)

Plasmid

#76428

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRPF4B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRPF4B

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-h-mmPtch1-B-NNQ-HA-GFP

Plasmid

#121164

Purpose

Expresses mmPtch1-B NNQ variant

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

Plasmid

#120909

Purpose

Expresses Ptch1-B (I766FV111FL114F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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