Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 12,055 results for: cel.2

Showing: 6801 - 6850 of 12055 results

  1. pcDNA4/TO-ORF66 C424A-2xStrep

    Plasmid
    #131110
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Use
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 424 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131110-plasmid-map-sequence-id-257473

    Enlarge

  8. LPAR2-DuET

    Plasmid
    #213330
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR2 (LPAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213330-plasmid-map-sequence-id-430864

    Enlarge

  9. GPRC5B-DuET

    Plasmid
    #213296
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC5B (GPRC5B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213296-plasmid-map-sequence-id-430840

    Enlarge

  11. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

    Enlarge

  13. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid
    #69115
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of 678-681aa region. Silent mutations i…
    Promoter
    CMV
    Available Since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69115-plasmid-map-sequence-id-122094

    Enlarge

  14. LTB4R2B-DuET

    Plasmid
    #213334
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LTB4R2B (LTB4R2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213334-plasmid-map-sequence-id-431823

    Enlarge

  15. CYSLTR2-DuET

    Plasmid
    #213226
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CYSLTR2 (CYSLTR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213226-plasmid-map-sequence-id-430788

    Enlarge

  17. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

    Enlarge

  18. pProk2.Cre.T2A.mCherry

    Plasmid
    #169013
    Purpose
    Expresses Cre under Prok2 minimal promoter in mammalian cells
    Depositor
    Michael Hastings
    Insert
    Cre Recombinase
    Use
    AAV and Cre/Lox
    Tags
    T2A and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Prokineticin 2
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169013-plasmid-map-sequence-id-337177

    Enlarge

  19. pcDNA3-His-SUMO2

    Plasmid
    #159324
    Purpose
    Mammalian cell expression of mouse His-SUMO2
    Depositor
    Jianjun Zhao
    Insert
    His-SUMO2 (Sumo2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159324-plasmid-map-sequence-id-311873

    Enlarge

  25. pD649-HAsp-IL1R2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156769
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1R2 (IL1R2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156769-plasmid-map-sequence-id-382023

    Enlarge

  43. INCTbiosyn-p35S(rc)2_4_5-GFP

    Plasmid
    #127520
    Purpose
    Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127520-plasmid-map-sequence-id-250826

    Enlarge

  45. pENTR-EUI

    Plasmid
    #111835
    Purpose
    Entry vector for adenoviral expression of Ecdysone receptor-based inducible transgene expression switch.
    Depositor
    Hyunju Ro
    Article
    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)
    Insert
    GAL4-minimal VP16-EcR (EcR Synthetic)
    Use
    Entry vector for expression
    Tags
    Expression
    Mutation
    Promoter
    CMV, 10XUAS
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111835-plasmid-map-sequence-id-218214

    Enlarge

Showing: 6801 - 6850 of 12055 results