Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,693 results for: tet on

Clear keywords
Showing: 6801 - 6850 of 7693 results

  10. p5B3-T

    Plasmid
    #92021
    Purpose
    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92021-plasmid-map-sequence-id-181444

    Enlarge

  17. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Judith Kimble
    Article
    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:
    Insert
    his-58, eGFP, mCherry
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171845-plasmid-map-sequence-id-337181

    Enlarge

  21. p5A1-Ti

    Plasmid
    #92018
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V, C151A
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92018-plasmid-map-sequence-id-190014

    Enlarge

  23. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92020-plasmid-map-sequence-id-190036

    Enlarge

  24. p5A3-T

    Plasmid
    #92019
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available Since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92019-plasmid-map-sequence-id-190012

    Enlarge

  31. pTKI-Tyr

    Plasmid
    #247647
    Purpose
    Expresses GFP-SUMO-Tyr-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Insert
    GFP-SUMO-Tyr-mCherry N-degron proteolysis dual-fluorescence reporter
    Use
    Mycobacterial integrating
    Tags
    HA tag and Myc tag
    Expression
    Bacterial
    Promoter
    PTet
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247647-plasmid-map-sequence-id-494850

    Enlarge

  32. pTKI-Ser

    Plasmid
    #247648
    Purpose
    Expresses GFP-SUMO-Ser-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Insert
    GFP-SUMO-Ser-mCherry N-degron proteolysis dual-fluorescence reporter
    Use
    Mycobacterial integrating
    Tags
    HA tag and Myc tag
    Expression
    Bacterial
    Promoter
    PTet
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247648-plasmid-map-sequence-id-494849

    Enlarge

  33. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

    Enlarge

  34. pCODE-3

    Plasmid
    #247475
    Purpose
    Plasmid for inducible expression of six tRNA encoding genes (argX, glyT, leuW, proL, argU, and ileX) in Escherichia coli, based on pSEVA121 backbone
    Depositor
    Morten Norholm
    Article
    Feller et al ACS Synth Biol. 2026 Apr 21. doi: 10.102
    Insert
    argX, glyT, leuW, proL, argU, and ileX
    Expression
    Bacterial
    Mutation
    Rearrangement of tRNA operon, tRNA flanking regio…
    Promoter
    pTet
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247475-plasmid-map-sequence-id-491679

    Enlarge

  35. mGreenLantern-[(A OR S) AND T] OR (C AND V)-SpyTag

    Plasmid
    #243789
    Purpose
    Expresses multifunctional construct enabling the [AND/(OR)]OR(AND)-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9), sortase eSrtA(4S9), TEV, HRV-3C, TEV and TVMV proteases. Construct contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mGreenLantern
    Tags
    6x His tag and SsrA
    Expression
    Bacterial
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243789-plasmid-map-sequence-id-486131

    Enlarge

  40. pET28-NFHΔ36-72

    Plasmid
    #232060
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 36-72
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232060-plasmid-map-sequence-id-464069

    Enlarge

  41. pET28-NFHΔ462-638

    Plasmid
    #232061
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 462-638
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232061-plasmid-map-sequence-id-464068

    Enlarge

  42. pET28-NFLtail

    Plasmid
    #232055
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFL tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Light tail domain
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232055-plasmid-map-sequence-id-464065

    Enlarge

  43. pET28-NFMtail

    Plasmid
    #232056
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    cDNA codon-optimized for E. coli; added N-termina…
    Promoter
    T7
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232056-plasmid-map-sequence-id-464064

    Enlarge

  44. pET28-NFMshuf1

    Plasmid
    #232058
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 1
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232058-plasmid-map-sequence-id-464062

    Enlarge

  46. pET28-NFMshuf2

    Plasmid
    #232059
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 2
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232059-plasmid-map-sequence-id-464059

    Enlarge

  48. p2H12ref

    Plasmid
    #221188
    Purpose
    cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    None (same as cdGreen) and Ptet
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221188-plasmid-map-sequence-id-437182

    Enlarge

  50. pMamCol1

    Plasmid
    #215184
    Purpose
    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli
    Depositor
    Krishna Kishore Inampudi
    Insert
    tsPurple
    Use
    AAV, CRISPR, and Synthetic Biology ; Recombinant …
    Tags
    SNAC-StrepII tag
    Expression
    Bacterial and Mammalian
    Promoter
    TetON 3G Bidirectional for Mammalian and Trc with…
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215184-plasmid-map-sequence-id-430375

    Enlarge

Showing: 6801 - 6850 of 7693 results