We narrowed to 35,392 results for: CaS

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MO91-dCas9-AID(P182X)

Plasmid

#83260

Purpose

Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

Depositor

Xing Chang

Article

Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

Insert

dCas9-AID P182X

Use

Retroviral

Tags

3xFLAG-NLS and FLAG

Expression

Mammalian

Mutation

D10A and H840A in Cas9; P182X in AID

Promoter

MSCV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LLP339 pGK-dCas9-SunTag

Plasmid

#100943

Purpose

dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-TdT

Plasmid

#126424

Purpose

Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Promoter

pCMV

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TetOn-dCas9-KRAB

Plasmid

#115545

Purpose

Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

Depositor

Rene Maehr

Article

Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

Type

Empty backbone

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Plasmid

#222991

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange-DEVD-mKate2

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9-SunTag(10X)-2A-BFP

Plasmid

#227389

Purpose

Adhesion module, dCas9-SunTag

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

dCas9-SunTag

Use

Lentiviral

Mutation

Enzymatically dead Cas9

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3M14nes

Plasmid

#157855

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-Caspase-1-mNeonGreen

Plasmid

#166784

Purpose

FRET analysis (pro caspase-1 cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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