We narrowed to 31,019 results for: uros

1. Direct-seq_lentiGuide-Puro-Tail-8A8G

   Plasmid

   #157981

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIVTR(T7A)-CRE-T2A-PuroR

   Plasmid

   #172298

   Purpose

   In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

   Depositor

   Patrik Verstreken

   Insert

   CRE-T2A-PuroR
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter A-initiating

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe puro H-Ras 12V (37G)

   Plasmid

   #12589

   Depositor

   Channing Der

   Article

   McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

   Insert

   HRas 12V 37G (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   12V, 37G

   Promoter

   Available since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pBRPy CAGGS-Mbd3-IRES-PURO

   Plasmid

   #52376

   Purpose

   Mammalian expression vector for constitutive expression of Mbd3

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Tags

   2xFlag (DYKDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKI-cjFOXL2-coemiRFP670-PGK-Puro

   Plasmid

   #186168

   Purpose

   Knock-in gene targeting in marmoset cells at FOXL2 locus

   Depositor

   Sho Yoshimatsu

   Article

   Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

   Insert

   emiRFP670
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSicoR-Ef1a-mCh-Puro-Luci

   Plasmid

   #31850

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   Luciferase RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

   Plasmid

   #163178

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-EglN2 H358A-pBabe-puro

   Plasmid

   #22705

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated cDNA: point mutation in codon 358 changi…

   Promoter

   Available since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-puro/Flag PD-L1 N200Q/N219Q

   Plasmid

   #121454

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N200Q/N219Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. F5A deltaCT S6K1 MSCV puro

    Plasmid

    #8464

    Depositor

    David Sabatini

    Article

    Ali et al J Biol Chem 2005 Apr 4;.

    Insert

    f5a delta ctp70 S6K1 (Rps6kb1 Rat)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deletion of 104 c-terminal amino acids mutation …

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCDF1-MCS2-EF1-Puro-RGS7

    Plasmid

    #113728

    Purpose

    Express RGS7 in mamalian cells

    Depositor

    Yardena Samuels

    Article

    Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

    Insert

    RGS7 (RGS7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCT-CMV-UBE3BΔHECT-copGFP-Puro

    Plasmid

    #107343

    Purpose

    Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B(ΔHECT)-copGFP (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    copGFP

    Expression

    Mammalian

    Mutation

    deletion of HECT domain (757-end)

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2

    Plasmid

    #183744

    Purpose

    Expresses Ki67 (shuffled Nterm)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (shuffled Nterm) (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    CD domain with shuffled 4 fragments fused to the …

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti X2 Puro/pTER shLUC (w589-1)

    Plasmid

    #21472

    Purpose

    3rd gen lentiviral Luciferase shRNA (H1/TO promoter), 3’LTR insertion, Puro

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly luciferase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-MAPKAP1Del-FHH-IRES-Puro

    Plasmid

    #120706

    Purpose

    Expresses MAPKAP1 Truncation-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Deleted amino acids 193-522

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBpuro-mNG-eDHFR(69K6)-p85iSH2

    Plasmid

    #172103

    Purpose

    Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe puro H-Ras 12V (40C)

    Plasmid

    #12590

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    HRas 12V 40C (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V, 40C

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

18. LA601: pMVP (L3-L2) P2A-Puro + WPRE

    Plasmid

    #121785

    Purpose

    pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Puro + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-3xFLAG-Puro PAK5 E144K

    Plasmid

    #167193

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 E144K

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 E144K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-3xFLAG-Puro PAK5 M173I

    Plasmid

    #167192

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 M173I

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 M173I
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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