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Halo-NEMO

Plasmid

#187899

Purpose

Mammalian cell expression of Halo-tagged NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

NEMO (Ikbkg Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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CCR4-DuET

Plasmid

#213200

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR4 (CCR4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-Lifeact-mKate2

Plasmid

#109493

Purpose

Muscle specific zebrafish expression of mKate2 fused to the actin binding lifeact sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-Lifeact-mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1333

Plasmid

#119268

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC120-MBP-DDX3X-monoGFP-His

Plasmid

#219985

Purpose

DDX3X(WT) protein purification

Depositor

Simon Alberti

Insert

DDX3X(WT) (DDX3X Human)

Tags

MBP and monoGFP and 6xHis

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry-SPASTIN

Plasmid

#180647

Purpose

Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, has siRNA resistance to GAACAGUGUG…

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc

Plasmid

#108234

Purpose

Broad host bacterial expression vector with constitutive Pc promoter.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-SB2

Plasmid

#228868

Purpose

PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB-NLS-2A-MLH1-SB

Use

CRISPR

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TC9pAB

Plasmid

#71310

Purpose

used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

Depositor

Karl Degenhardt

Insert

Cas9

Use

In vitro transcription

Tags

NLS

Promoter

T7

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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