162,309 results

1. T7-TPase

   Plasmid

   #51818

   Purpose

   For in vitro transcription of Tol2 transposase using T7 Promoter

   Depositor

   Elly Tanaka

   Article

   Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

   Insert

   Tol2 Transposase
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   April 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLNCX2-mEGFP-CHMP2B

   Plasmid

   #115329

   Purpose

   expresses mEGFP-CHMP2B in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   CHMP2B (CHMP2B Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Ptet-RFP-shR-rtTA

   Plasmid

   #35625

   Purpose

   Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

   Depositor

   Howard Gu

   Article

   Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

   Type

   Empty backbone

   Use

   AAV and RNAi; Tet inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ptet

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-MBP-2xNLS-tdTomato

   Plasmid

   #104054

   Purpose

   An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-tdTomato
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   MBP

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85006

   Purpose

   For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   EGFP-ires-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Pepper-fused sgRNA

   Plasmid

   #217520

   Purpose

   Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   Pepper-fused sgRNA for spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSSRB069_pAC8-hsDDB1dB

   Plasmid

   #218790

   Purpose

   Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Deleted amino acids 396-705 and replaced with GNG…

   Promoter

   polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1 mito-TagRFP-T

   Plasmid

   #174543

   Purpose

   labels mitochondria with TagRFP-T

   Depositor

   Minna Roh-Johnson

   Article

   Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

   Insert

   MTS-tagRFP-T
   

   Use

   Lentiviral

   Tags

   tagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tet_pLKO.1_puro_shNRF2 #1

    Plasmid

    #136584

    Purpose

    Expresses an inducible short hairpin targeting human NRF2 sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shNRF2v1 (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge