We narrowed to 156,007 results for: ins

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Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-p40R57Q PX

Plasmid

#19009

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

aa1-148 (PX domains) site directed mutagenesis: …

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pHelper_TetR/pTet-Tnpase (carbR)

Plasmid

#213911

Purpose

ATc-inducible himar1C9 transposase expression - carb/amp resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pC4EN-F1-mGAF670 (FKBP-mGAF670)

Plasmid

#80003

Purpose

miRFP670 GAF domain fused to FKBP

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FKBP

Tags

GAF domain of miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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PKC gamma CAT

Plasmid

#21238

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-337

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pSDH61

Plasmid

#139033

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (no STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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CMV566 A-USF

Plasmid

#33360

Depositor

Charles Vinson

Article

Qyang et al Mol Cell Biol. 1999 Feb;19(2):1508-17.

Insert

USF (Usf1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant Negative (see notes below)

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
PSUPER-344(hPot1)

Plasmid

#11133

Depositor

Christopher Counter

Article

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against human Protection of Telomeres 1 (POT1 Human)

Use

RNAi

Expression

Mammalian

Mutation

N/A

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDM1501

Plasmid

#108997

Purpose

safe haven knock-in plasmid for the act5 locus

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-D91G

Plasmid

#47646

Purpose

pAC-EsaR with a a272g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-D91G

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Aspartic acid 91 to Glycine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
mTB3-Myc tag

Plasmid

#210205

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

Myc-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB-ITSN1

Plasmid

#114425

Purpose

Translocating ITSN1 to iLID and activate Cdc42

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

ITSN1

Tags

mScarlet

Expression

Mammalian

Mutation

truncated ITSN1 (1159-1509)

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1046

Plasmid

#108989

Purpose

Inducible expression vector (dox-on), N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCS-PluxIlux

Plasmid

#47641

Purpose

wt luxI promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PluxI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PluxI

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pDM1021

Plasmid

#108978

Purpose

shuttle vector, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-uniRapR-P-Vav2(DPZ)

Plasmid

#91875

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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JAF99.4

Plasmid

#45560

Depositor

Gregory Pazour

Article

Follit et al J Cell Biol. 2010 Jan 11;188(1):21-8. do

Insert

Pkhd1 (Pkhd1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains only the cytoplasmic tail (last 193 amin…

Promoter

CMV

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLSLZ.D.R

Plasmid

#51510

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Promoter

2x35S

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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CRY2clust-eGFP-2xC2-iLID

Plasmid

#114420

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1020

Plasmid

#108977

Purpose

shuttle vector, C-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

mCherry

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB-Tiam1

Plasmid

#114424

Purpose

Translocating Tiam1 to iLID and activate Rac1

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

Tiam1

Tags

mScarlet

Expression

Mammalian

Mutation

truncated Tiam1 (64-437)

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PKC beta II dNPS

Plasmid

#16383

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
iLID-eGFP-2xC2-iLID

Plasmid

#114418

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-V220A

Plasmid

#47645

Purpose

pAC-EsaR with a c659t mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-V220A

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Valine 220 to Alanine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only