We narrowed to 144,718 results for: ins

1. pDM344

   Plasmid

   #108974

   Purpose

   shuttle vector

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Dictyostelium discoideum shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sirius/pRSETB

   Plasmid

   #51956

   Purpose

   bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

   Insert

   Sirius
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor_Tn-pOUT

   Plasmid

   #213905

   Purpose

   Mariner transposon with outward-facing pJ23104 promoter

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and outward-facine pJ23104 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETA-PAGFP

   Plasmid

   #11911

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Insert

   pRSETA-PAGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The PAGFP has four mutations (L64F/T65S/V163A/T20…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC delta DN

   Plasmid

   #16389

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC delta isoform 2 (Prkcd Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Dominant negative (kinase inactive): K376R

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AICSDP-82:SON-mEGFP

   Plasmid

   #133964

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON

   Depositor

   Allen Institute for Cell Science

   Insert

   SON Homology Arms with mEGFP-linker (SON Human)
   

   Use

   CRISPR

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. V5-BS2-NLS (Nucleoplasmin)

   Plasmid

   #220342

   Purpose

   Mammalian expression vector BS2 in nucleus

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   NLS (Nucleus localization sequence) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. V5-BS2-NES (Cytosol)

   Plasmid

   #220341

   Purpose

   Mammalian expression vector BS2 in cytoplasm

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Use

   Tags

   NES (Nuclear exclusion sequence) and V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDonor_Tn[sfGFP]

    Plasmid

    #213902

    Purpose

    Mariner transposon expressing sfGFP with kan selection casette

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PKC delta CAT

    Plasmid

    #16388

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta (Prkcd Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain, aa 334-674

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. V5-BS2-NIK3x (Nucleolus)

    Plasmid

    #220346

    Purpose

    Mammalian expression vector BS2 in nucleolus

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    Nik3x (Nucleolus localization tag) and V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pcDNA3.1 3xF-NHP2

    Plasmid

    #126871

    Purpose

    Expresses 3xFlag NHP2

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    NHP2 (NHP2 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVITRO1-SS-338

    Plasmid

    #68795

    Purpose

    Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    C-Terminal Sensor 1 V1
    mCherry
    ZF9 Op x6
    EGFP
    N-Terminal Sensor 1 V2
    

    Use

    Tags

    IRES, NLS, flag, and nls

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PKC beta II DN

    Plasmid

    #16385

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta II (Prkcb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (kinase inactive): K371R

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PKC alpha dNPS

    Plasmid

    #21233

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-29

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

17. AICSDP-116:PCNA-mEGFP

    Plasmid

    #170818

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

    Depositor

    Allen Institute for Cell Science

    Insert

    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. Mito-V5-BS2 (Mitochondrial matrix)

    Plasmid

    #220344

    Purpose

    Mammalian expression vector BS2 in mitocondria

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    MTS-matrix and V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Filamin-C-stFRET

    Plasmid

    #61107

    Purpose

    Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

    Depositor

    Fred Sachs

    Article

    Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

    Insert

    filamin-stFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCIS-krPAM1

    Plasmid

    #25456

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM1 (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PKC delta dNPS

    Plasmid

    #16387

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta (Prkcd Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: aa 15…

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

23. PSUPER-344(hPot1)

    Plasmid

    #11133

    Depositor

    Christopher Counter

    Article

    Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

    Insert

    RNAi against human Protection of Telomeres 1 (POT1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

24. CelTag Plasmid

    Plasmid

    #66562

    Purpose

    Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

    Depositor

    David Engelke

    Article

    Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

    Insert

    CelTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPICZA_CeTRIC-B1_Del

    Plasmid

    #87344

    Purpose

    Inducible expression of CeTRIC-B1 in P.Pichia strains

    Depositor

    Zhenfeng Liu

    Article

    Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

    Insert

    TRIC-B1
    

    Use

    Tags

    polyhistidine

    Expression

    Yeast

    Mutation

    Δ248-297

    Promoter

    AOX1

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PKC gamma CAT

    Plasmid

    #21238

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC gamma (Prkcg Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-337

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Filamin-M-stFRET

    Plasmid

    #61105

    Purpose

    Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

    Depositor

    Fred Sachs

    Article

    Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

    Insert

    filamin-stFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. UIMLx2

    Plasmid

    #174888

    Purpose

    UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

    Depositor

    Peter Kaiser

    Article

    Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

    Insert

    Met4 UIML
    

    Use

    Tags

    Avi-6xHis-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Actinin-M-stFRET

    Plasmid

    #61103

    Purpose

    Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

    Depositor

    Fred Sachs

    Article

    Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

    Insert

    α-actinin-stFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge