We narrowed to 14,204 results for: RING;
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Plasmid#137089PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)DepositorInsert4*(GFP11)
UsePcr template for donor dnaExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB035a
Plasmid#79190PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
ExpressionBacterialPromoterPlac62; PtetAvailable SinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
ExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAP1836
Plasmid#137087PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherryDepositorInsertmCherry
UsePcr template for donor dnaExpressionBacterialAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
TagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSIBR005
Plasmid#177668PurposeConstitutive expression of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIBR001
Plasmid#177628PurposeSIBR Intron 1 (Int1) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
TagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR1
Plasmid#183289PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR1 geneDepositorInsertFGFR1
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR2
Plasmid#183290PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR2 geneDepositorInsertFGFR2
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMV-ycrtI
Plasmid#65342Purposesysthesis of crtI gene in the beta-carotene pathwayDepositorInsertycrtI
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGE-attBCC-FRT-mW (pTL788)
Plasmid#115159PurposeIntegration vector for phiC31-mediated transgenesis using a genomic attP-CC site. Contains an attB-CC site, the visible GMR-white flanked by a FRT site for FLP-mediated removal.DepositorInsertattB-CC FRT
ExpressionInsectAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPI180
Plasmid#113222Purposegeneric knockin vector for C-terminal tagging with GFPDepositorTypeEmpty backboneUseGeneric knockin vector for c-terminal tagging wit…TagsGFPAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-C
Plasmid#170154PurposeAAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertRESCUE-DDC-λN-NES
UseAAVExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.540
Plasmid#184959PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
ExpressionBacterialMutationfabH donor G954A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_ATP1A1_G7
Plasmid#173204PurposeExpresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.DepositorInsertATP1A1 G7 sgRNA + SpCas9
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB6679
Plasmid#106158PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_EGFR
Plasmid#183285PurposeAll-in-One CRISPRko system with a guide RNA that targets EGFR geneDepositorInsertEGFR
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEJS507-pCDest2-noAcr-mTagBFP2-IRES
Plasmid#85748PurposeExpresses mTagBFP2 only; used as a controlDepositorInsertmTagBFP2
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A
Plasmid#129679PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationD570A; AaCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSuper.Retro.Puro-WDR5 shRNA 4
Plasmid#59977PurposeKnockdown of WDR5DepositorInsertWDR5 shRNA
UseRetroviralAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-mZFP57
Plasmid#87224PurposeExpress hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
SB53
Plasmid#21488DepositorAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG
Plasmid#29583DepositorInsertphosphatidylinositol phosphate kinase type I gamma
TagsFlag and GFPExpressionMammalianMutationN/AAvailable SinceFeb. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
PMV-ycrtYB
Plasmid#65343Purposesysthesis of crtYB gene in the beta-carotene pathwayDepositorInsertycrtYB
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange
Plasmid#149691PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianPromoterhSynAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_SRC
Plasmid#183322PurposeAll-in-One CRISPRko system with a guide RNA that targets SRC geneDepositorInsertSRC
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL2
Plasmid#183268PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL2 geneDepositorInsertABL2
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL1
Plasmid#183267PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL1 geneDepositorInsertABL1
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcry:GFP,-503unc:hACTA1GFP
Plasmid#172529PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.DepositorInsertATCA1
ExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRExpressionWormAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtPHYB-S_X
Plasmid#131183PurposeExpresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtPHYB-S
ExpressionMammalianPromoterCMVAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_NcVVD-LOV_X
Plasmid#131176PurposeExpresses NcVVD-LOV (Neurosporra crassa) followed by restriction sitesDepositorInsertNcVVD-LOV
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS/KS-mId4sp
Plasmid#16046DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1360 - Intron GG3 - smu-1
Plasmid#159806PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1358 - Intron GG1 - smu-1
Plasmid#159804PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - smu-1
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFUSE-B_P2rP4
Plasmid#97185PurposeFor fusion gene cloning. Recombine Gene B into this Gateway Entry VectorDepositorTypeEmpty backboneUseGatewayAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_VPR_EYFP
Plasmid#167890PurposePiggyBac compatible plasmid expressing Cas9-VPRDepositorInsertCas9-VPR
UseCRISPRAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV CDHblast Rev(V16D)-EGFP-V5
Plasmid#175165PurposeLentiviral expression of Rev(V16D)-EGFP-V5DepositorInsertrev
UseLentiviralTagsV5ExpressionMammalianMutationV16DAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMV-ycrtE
Plasmid#65341Purposesysthesis of crtE gene in the beta-carotene pathwayDepositorInsertycrtE
UseSynthetic BiologyAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC0.062
Plasmid#119651PurposeLevel 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.DepositorInsertBba_B0011
UseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_EYFP
Plasmid#167866PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV Sbf-Sal linker
Plasmid#50434PurposeCloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Geph UTR shRNA
Plasmid#121070PurposeshRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNADepositorInsertGPHN shRNA (Gphn Rat)
UseRNAiAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTL472
Plasmid#69882PurposedCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassetteDepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSuper.Retro.Puro-PKN1 shRNA 4
Plasmid#59978PurposeKnockdown of PKN1DepositorInsertPKN1 shRNA
UseRetroviralAvailable SinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only