Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 18,363 results for: Tec

Showing: 6851 - 6900 of 18363 results

  2. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60343-plasmid-map-sequence-id-96621

    Enlarge

  3. pOPTXcGMPRELUC

    Plasmid
    #68503
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…
    Promoter
    OPTX promoter with cGMPRE
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

    Plasmid
    #160635
    Purpose
    TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4
    Insert
    AcrIIA4
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    Sept. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    160635-plasmid-map-sequence-id-427149

    Enlarge

  14. pCEP4-HA-Flag-natT1R2 ECD

    Plasmid
    #113954
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    residues 22-568
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113954-plasmid-map-sequence-id-215168

    Enlarge

  27. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

    Enlarge

  28. pOCC194

    Plasmid
    #118882
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    SNAP tag, cleavable with TEV protease, HIS6, clea…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118882-plasmid-map-sequence-id-232359

    Enlarge

  29. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

    Enlarge

  30. pOCC231

    Plasmid
    #118885
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and mCitrine…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118885-plasmid-map-sequence-id-232309

    Enlarge

  34. pOPTXGARE

    Plasmid
    #68542
    Purpose
    cyclic nucleotide reporter for bacteria or plant cells
    Depositor
    Helen Irving
    Article
    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1
    Insert
    OPTX promoter with GARE (AT1G33440 Mustard Weed)
    Use
    Tags
    luciferase
    Expression
    Bacterial and Plant
    Mutation
    5x GARE inserted 190 bp 5' pf ATG (GARE = ta…
    Promoter
    OPTX promoter with GARE
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68542-plasmid-map-sequence-id-121994

    Enlarge

  41. pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50421
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Article
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50421-plasmid-map-sequence-id-79593

    Enlarge

  42. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63785-plasmid-map-sequence-id-106471

    Enlarge

  44. PatoG-OG241

    Plasmid
    #72839
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoG
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72839-plasmid-map-sequence-id-133603

    Enlarge

Showing: 6851 - 6900 of 18363 results